MABF3024

Anti-Mayaro Virus E1 Antibody, clone MAY-115

• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: MAYV-115, monoclonal
• peso molecular: calculated mol wt 137.06 kDa
• purificado por: using protein G
• reatividade de espécies: virus
• embalagem: antibody small pack of 100 μL
• técnica(s): ELISA: suitable; neutralization: suitable
• Isotipo: IgG2κ
• sequência de epítopo: N-terminal extracellular domain
• nº de adesão UniProt: DQ487410
• Condições de expedição: 2-8°C
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

Structural polyprotein (UniProt: Q8QZ72; also known as p130) is encoded by the MAYVgp3 gene (Gene ID: 935141) in Mayaro virus. Following its synthesis it is cleaved into the following six chains: Capsid protein (aa 1-258), Precursor of protein E3/E2 (aa 259-746), Assembly protein E3 (aa 259-324), Spike glycoprotein E2 (aa 325-746), 6K protein (aa 747-806), and Spike glycoprotein E1 (aa 807-1242). Mayaro virus (MAYV) is a mosquito-transmitted arthritogenic alphavirus in the Togaviridae family of positive-stranded RNA viruses. Its envelope glycoproteins associate in the endoplasmic reticulum and form a heterotrimer comprised of the E3, E2, and E1 proteins. E2 is a single-pass type I membrane protein that plays a role in viral attachment to target host cell, by binding to the cell receptor. It is synthesized as a p62 precursor (precursor of protein E3/E2), which is then processed by furin at the cell membrane just before virion budding, giving rise to E2-E1 heterodimer. The p62-E1 heterodimer is shown to be stable, whereas E2-E1 is unstable and dissociates at low pH. It has been reported that p62 is processed at the last step, presumably to avoid E1 fusion activation before its final export to cell surface. The C-terminus region of E2 contains a transitory transmembrane region that is disrupted by palmitoylation and results in reorientation of the C-terminal tail from lumenal to cytoplasmic side. Clone MAY-115 offers protection against lethal effects of MAYV in a murine model when injected one day prior to virus inoculation. However, its protective effects are significantly reduced when injected two days post infection. (Ref.: Earnest, JT., et al. (2019). J. Exp. Med. 216(10); 2282-2301).

Especificidade

Clone MAYV-115 is a mouse monoclonal antibody that detects E1 glycoprotein in Mayaro virus.

Imunogênio

Mayaro virus, CH strain and boosted with recombinant MAYV E2 protein mixed with incomplete Freund′s adjuvant.

Aplicação

Quality Control Testing

Evaluated by ELISA with recombinant MAYV E1 protein.

ELISA Analysis: Various dilutions of this antibody detected recombinant E1 protein of Mayaro virus CH (strain Peru).

Tested Applications

Neutralizing Analysis: A representative lot neutralized Mayarao virus and protected mice from lethal virus challenge. (Earnest, J.T., et al. (2019). J Exp Med.;216(10):2282-2301).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Mayaro Virus E1, clone MAY-115 , Cat. No. MABF3024, is a mouse monoclonal antibody that detects Mayaro virus E1 glycoprotein and is tested for use in ELISA and Neutralizing Activity.

forma física

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable