MABF2225

Anti-HSV-2 VP16 Antibody, clone LP1

• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: LP1, monoclonal
• peso molecular: calculated mol wt 54.6 kDa; observed mol wt ~37 kDa
• purificado por: using protein G
• reatividade de espécies: herpes simplex virus 1
• embalagem: antibody small pack of 100 μL
• técnica(s): immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1
• sequência de epítopo: Unknown
• Condições de expedição: 2-8°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: mouse ... vp16> VP16(1487335)

Descrição

Descrição geral

Alpha trans-inducing protein (UniProt: Q69113; also known as Alpha-TIF) is encoded by the VP16 (also known as UL48) gene (Gene ID: 1487335) in human Herpes Simplex virus 2. Herpes simplex virus (HSV) is a large enveloped DNA virus that replicates in the nuclei of mammalian cells. Its virion contains two regulatory proteins that are reported to modulate gene expression in infected cells: the transcriptional activator VP16 and the virion host shutoff protein vhs. VP16, an abundant structural protein, is shown to be essential for virion assembly and it stimulates transcription of the HSV immediate-early (IE) genes. Its transcriptional activation domain is localized in the C-terminal region. It is targeted to the TAATGARATTC consensus sequence found in IE promoters through interactions with the host factors Oct-1 and HCF. It is delivered by the infecting virion and acts during the earliest stages of infection to stimulate transcription of the viral IE genes, thereby facilitating the onset of the lytic program of viral gene expression. It is also reported to suppress vhs host protein synthesis and induce accelerated turnover of cellular and viral mRNAs. Clone LP1 is directed against VP16 in both HSV-1 and HSV-2. (Ref.: Smibert, CA., et al. (1994). J. Virol. 68(4); 2339-2346; McLean, C., et al. (1982). J. Gen. Virol. 63(2); 297-305).

Especificidade

Clone LP1 is a mouse monoclonal antibody that detects VP16 protein of Herpes simplex virus 2 (HSV-2) and displays cross reactivity with HSV-1.

Imunogênio

Herpes simplex virus -2; strain 25766.

Aplicação

Quality Control Testing

Evaluated by Western Blotting in lysate from HSV-1 infected Vero cells.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected VP16 protein in lysate from HSV-1 infected Vero cells.

Tested Applications

Immunoprecipitation Analysis): A representative lot immunoprecipitated HSV-2 VP16 in Immunoprecipitation applications (McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).

Western Blotting Analysis: A representative lot detected HSV-2 VP16 in Western Blotting applications (Crameri, M., et al. (2018). Nat Commun.; 9(1):1980; McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).

Immunofluorescence Analysis: A representative lot detected HSV-2 VP16 in Immunofluorescence applications (McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-HSV-2 VP16, clone LP1, Cat. No. MABF2225, is a mouse monoclonal antibody that detects Alpha trans-inducing protein (VP16) and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

forma física

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable