MABF1986M

Anti-NKR-P1G Antibody, clone ADA3

clone ADA3, from hamster(Armenian)

• Sinônimo(s): Killer cell lectin-like receptor subfamily B member 1, Immunoreceptor NKR-P1E, Killer cell lectin-like receptor subfamily B member 1G, Natural killer cell surface protein NKR-P1G, Natural killer lectin-like receptor 1E
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: hamster (Armenian)
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: ADA3, monoclonal
• reatividade de espécies: rat
• embalagem: antibody small pack of 25 μL
• técnica(s): flow cytometry: suitable; immunoprecipitation (IP): suitable
• Isotipo: IgG
• nº de adesão NCBI: NP_001078874.1
• nº de adesão UniProt: Q0ZUP0
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: rat ... Klrb1(689817)

Descrição

Descrição geral

Killer cell lectin-like receptor subfamily B member 1 (UniProt: Q0ZUP0; also known as Immunoreceptor NKR-P1E, Killer cell lectin-like receptor subfamily B member 1G, Natural killer cell surface protein NKR-P1G, Natural killer lectin-like receptor 1E) is encoded by the Klrb1 (also known as Klrb1d, Klrb1g, Klrb6, Nkrp1e, Nkrp1g) gene (Gene ID: 689817) in rat. NKR-P1G is a single-pass type II membrane protein with a cytoplasmic domain (aa 1-42), a helical domain (aa 43-63), and an extracellular domain (aa 64-214). NKR-P1G is expressed by subsets of lymphocytes with highest prevalence in gut and liver mucosa. The number of NKR-P1G+ cells are shown to increase with age and its expression level is modulated by cytokines, with an increase induced by inflammatory cytokines. It is shown to be mainly expressed by Ly49s3+ cells. It is reported to inhibit cytotoxicity and calcium responses in IL-2-activated NK cells. NKR-P1G is a potent inhibitory receptor for a panel of Clr ligands. The intracellular domain of NKR-P1G contains an immunoreceptor tyrosine-based inhibitory motif that is phosphorylated by Src-kinases upon activation. This allows recruitment of phosphotyrosine phosphatases, which in turn negatively regulates the signaling cascades that is essential for cytotoxicity and cytokine production. (Ref.: Kveberg, L et al (2015). Eur J Immunol. 45(2); 501-512).

Especificidade

Clone ADA3 is an Armenian hamster monoclonal antibody that detects Killer cell lectin-like receptor subfamily B member 1 (NKR-P1G) in rat. It targets an epitope within the extracellular domain.

Imunogênio

Chinese hamster ovary (CHO) cells stably transfected with FLAG-tagged extracellular portion of NKR-P1G.

Epitope: extracellular domain

Aplicação

Anti-NKR-P1G, clone ADA3, Cat. No. MABF1986 is an Armenian hamster monoclonal antibody that detects NKG-P1G and has beent tested for use in Flow Cytometry and Immunoprecipitation.

Immunoprecipitation Analysis: A representative lot detected NKR-PIG in Immunoprecipitation applications (Kveberg, L., et. al. (2015). Eur J Immunol. 45(2):501-12).

Flow Cytometry Analysis: A representative lot detected NKR-PIG in Flow Cytometry applications (Kveberg, L., et. al. (2015). Eur J Immunol. 45(2):501-12).

Research Category
Inflammation & Immunology

Qualidade

Evaluated by Flow Cytometry in Ltk (mouse fibroblast) cells stably transfected with FLAG-tagged rat NKR-P1G.

Flow Cytometry Analysis: 1 µg of this antibody detected NKR-P1G in Ltk (mouse fibroblast) cells stably transfected with FLAG-tagged rat NKR-P1G.

Descrição-alvo

23.96 kDa calculated.

forma física

Format: Purified

Protein G purified

Purified Armenian hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1