ABE498

Anti-Lysine-specific demethylase 7 Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): Lysine-specific demethylase 7, JmjC domain-containing histone demethylation protein 1D
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• reatividade da espécie (prevista por homologia): chimpanzee, giant panda, mouse, canine, rhesus macaque, marmoset
• técnica(s): western blot: suitable
• nº de adesão NCBI: NP_085150
• nº de adesão UniProt: Q6ZMT4
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... JHDM1D(80853)

Descrição

Descrição geral

Lysine-specific demethylase 7, also known as JmjC domain-containing histone demethylation protein 1D, and encoded by the gene JHDM1D/KDM7/KIAA1718, is an important histone demethylase particularly for brain development. JHDM1D specifically demethylates dimethylated ′Lys-9′ and ′Lys-27′ (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 ′Lys-20′ residue (H4K20Me1), thereby playing a central role in histone code. JHDM1D also specifically binds trimethylated ′Lys-4′ of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. JHDM1D also demethylates H3K9me2 in absence of H3K4me3 and JHDM1D has activity toward H4K20Me1 only when the nucleosome is used as a substrate and when not the histone octamer is used as substrate. JHDM1D is located in the nucleus and widely expressed but lowered expression is observed in glial cells and lymphoid cells. JHDM1D demonstrates tumor suppression by regulating angiogenesis factors when overexpression under starvation conditions in certain cancers, thus JHDM1D is being examined as a potential anti-cancer target.

Especificidade

This antibody is expected to cross react with two known isoforms of Lysine-specific demethylase 7 based upon immunogen design.

Imunogênio

KLH-conjugated linear peptide corresponding to human Lysine-specific demethylase 7.

Aplicação

Detect Lysine-specific demethylase 7 using this rabbit polyclonal antibody, Anti-Lysine-specific demethylase 7 Antibody validated for use in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Qualidade

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Lysine-specific demethylase 7 in 10 µg of HEK293 cell lysate.

Descrição-alvo

~110 kDa observed. Uniprot describes two isoforms at 107 kDa (isoform 1) and 92 kDa (isoform 2).

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable