574597-M

Anti-Superoxide Dismutase (Cu/Zn Enzyme) Sheep pAb

liquid, Calbiochem^®

• Sinônimo(s): Anti-SOD
• Código UNSPSC: 12352203

Propriedades

• fonte biológica: sheep
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• forma: liquid
• contém: ≤0.1% sodium azide as preservative
• reatividade de espécies: mouse, rat, human, monkey
• fabricante/nome comercial: Calbiochem^®
• disponibilidade: not available in Canada,
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• Isotipo: IgG
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

Recognizes Cu/Zn SOD in red blood cells. Does not cross-react with Mn SOD.

Sheep polyclonal antibody purified by DEAE ion-exchange chromatography. Recognizes the Cu/Zn superoxide dismutase protein.

Superoxide dismutase Cu/Zn enzyme (SOD (Cu/Zn)) is a homodimeric metalloenzyme of aerobic organisms, comprised of subunits of ~16 kDa. Found in a variety of cell types, including erythrocytes, liver, and brain, it serves to protect cells against the oxidative damage of free radicals by catalyzing the conversion of superoxide anions (O₂-) to molecular oxygen (O₂) and hydrogen peroxide (H₂O₂).

This Anti-Superoxide Dismutase Sheep pAb is validated for use in WB, Immunoelectrophoresis, Frozen sections, Paraffin sections, Free-floating sections for the detection of Superoxide Dismutase.

Imunogênio

Human

highly purified, human erythrocyte SOD Cu/Zn.

Aplicação

Immunoblotting (1:1000)

Immunoelectrophoresis (see comments)

Frozen sections (see comments)

Paraffin sections (see comments)

Free-floating sections (see comments)

Embalagem

Please refer to vial label for lot-specific concentration.

Advertência

Toxicity: Standard Handling (A)

forma física

In glycine-buffered saline, pH 7.4.

Reconstituição

Following initial thaw, aliquot and freeze (-20°C).

Nota de análise

Positive Control
Human red blood cell lysate

Outras notas

Pasinelli, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 15763.

This antibody is monospecific as determined by immunoelectrophoresis against human red blood cell lysate and confirmed by immunodiffusion against a human red blood cell lysate and a known anti-SOD (Cu/Zn) antibody. Does not exhibit cross-reactivity with SOD (Mn) by double diffusion or with human serum. This antibody is reported to be suitable for use with free-floating, frozen, or paraffin embedded sections when used in immunohistochemistry. Variables assocated with assay conditions will dictate the proper working dilution.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable