391334

BES

ULTROL^® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer

• Sinônimo(s): BES, Free Acid, ULTROL^® Grade, N,N- bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
• Fórmula empírica (Notação de Hill): C₆H₁₅NO₅S
• Número CAS: 10191-18-1
• Peso molecular: 213.25
• Número MDL: MFCD00007533
• Código UNSPSC: 12161700
• NACRES: NA.25

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥99% dry basis (titration)
• forma: solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• cor: white
• pKa (20 °C): 7.12
• solubilidade: water: 1 mg/mL
• traços de cátion: heavy metals: ≤1 ppm
• Condições de expedição: ambient
• temperatura de armazenamento: 15-25°C
• InChI: 1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
• chave InChI: AJTVSSFTXWNIRG-UHFFFAOYSA-N

Descrição

Descrição geral

Absorbance (100 mM, 260 nm):≤0.05.

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pK_a of 7.12 at 20°C.

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.

Advertência

Toxicity: Standard Handling (A)

Reconstituição

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.

Outras notas

De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
Chen, C.A., and Okayama, H. 1988. Biotechniques6, 632.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable