Novel assay of antibacterial components in manuka honey using lucigenin-chemiluminescence-HPLC.

Novel assay of antibacterial components in manuka honey using lucigenin-chemiluminescence-HPLC.

Analytica chimica acta (2017-01-14)

Koji Karasawa, Shiomi Haraya, Sachie Okubo, Hidetoshi Arakawa

ABSTRACT

Five components (hydrogen peroxide, methylglyoxal, dihydroxyacetone, fructose and glucose) of New Zealand manuka honey (Leptospermum scoparium) were analyzed using lucigenin chemiluminescence high-performance liquid chromatography (lucigenin-CL-HPLC). We focused on active oxygen species produced from the components in order to easily detect these five components contained in manuka honey. H

MATERIALS

Product Number

Brand

Product Description

245615

Sigma-Aldrich

2-Bromo-4′-nitroacetophenone, 95%

Pricing and availability is not currently available.

75707

Sigma-Aldrich

Bis(2,4,6-trichlorophenyl) oxalate, BioReagent, suitable for chemiluminescence, ≥99.0% (AT)

Pricing and availability is not currently available.