Lentivirus-mediated silencing of spindle and kinetochore-associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells.

Lentivirus-mediated silencing of spindle and kinetochore-associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells.

Molecular medicine reports (2015-01-13)

Xiujuan Shi, Xianzhen Chen, Hu Peng, E Song, Ting Zhang, Junxiang Zhang, Jing Li, Himaya Swa, Yongxin Li, Sekwon Kim, Xiaoqing Liu, Chen Zhang

PMID25573192

ABSTRACT

Spindle and kinetochore‑associated protein 1 (SKA1) is an important component of the human kinetochore, which plays a key role in mitosis. The resent study was designed to investigate the role of SKA1 in human glioblastoma. The results of the present study demonstrated that SKA1 was expressed in human glioblastoma cells. In addition, the knockdown of SKA1 expression in the A172 and U251 human glioblastoma cell lines was accomplished using a lentivirus infection method. An MTT assay demonstrated that downregulation of SKA1 may inhibit cell proliferation, without affecting the cell cycle. Furthermore, knockdown of SKA1 expression resulted in reduced cell invasion. The results of the present study indicated that SKA1 may be a potential target protein for antiproliferative and anti‑invasive therapeutic strategies of human glioblastoma.

MATERIALS

Product Number

Brand

Product Description

P4170

Sigma-Aldrich

Propidium iodide, ≥94.0% (HPLC)

M5655

Sigma-Aldrich

Thiazolyl Blue Tetrazolium Bromide, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

M2128

Sigma-Aldrich

Thiazolyl Blue Tetrazolium Bromide, 98%

74255

Supelco

Sodium dodecyl sulfate, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)

71725

Sigma-Aldrich

Sodium dodecyl sulfate, BioUltra, for molecular biology, ≥99.0% (GC)

RDD040

Sigma-Aldrich

Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC), free-flowing, Redi-Dri^™

L3771

Sigma-Aldrich

Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

717975

Sigma-Aldrich

Methanol, NMR reference standard

W292907

Sigma-Aldrich

Isopropyl alcohol, ≥99.7%, FCC, FG

62862

Sigma-Aldrich

Sodium dodecyl sulfate, ≥90% ((Assay))

L4509

Sigma-Aldrich

Sodium dodecyl sulfate, ReagentPlus^®, ≥98.5% (GC)

436143

Sigma-Aldrich

Sodium dodecyl sulfate, ACS reagent, ≥99.0%

L6026

Sigma-Aldrich

Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)

71729

Sigma-Aldrich

Sodium dodecyl sulfate, ≥98.0% (GC)

75746

Sigma-Aldrich

Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets

71717

Sigma-Aldrich

Sodium dodecyl sulfate, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate

322415

Sigma-Aldrich

Methanol, anhydrous, 99.8%

L5750

Sigma-Aldrich

Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis

P4864

Sigma-Aldrich

Propidium iodide solution

493511

Sigma-Aldrich

Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%

59304

Sigma-Aldrich

2-Propanol, BioUltra, for molecular biology, ≥99.5% (GC)

721956

Sigma-Aldrich

Methanol solution, NMR reference standard, 4% in methanol-d₄ (99.8 atom % D), NMR tube size 3 mm × 8 in.

71736

Sigma-Aldrich

Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 10% in H₂O

I9516

Sigma-Aldrich

2-Propanol, BioReagent, for molecular biology, ≥99.5%

675431

Sigma-Aldrich

2-Propanol, HPLC Plus, for HPLC, GC, and residue analysis, 99.9%, poly coated bottles

05030

Sigma-Aldrich

Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 20% in H₂O

459836

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%

49419

Sigma-Aldrich

L-Glutamine, BioUltra, ≥99.5% (NT)

G3126

Sigma-Aldrich

L-Glutamine, ReagentPlus^®, ≥99% (HPLC)

G8540

Sigma-Aldrich

L-Glutamine, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source