- Exploring the human mesenchymal stem cell tubule communication network through electron microscopy.
Exploring the human mesenchymal stem cell tubule communication network through electron microscopy.
Ultrastructural pathology (2014-10-01)
Sabrina Valente, Roberta Rossi, Leonardo Resta, Gianandrea Pasquinelli
PMID25268461
ABSTRACT
Cells use several mechanisms to transfer information to other cells. In this study, we describe micro/nanotubular connections and exosome-like tubule fragments in multipotent mesenchymal stem cells (MSCs) from human arteries. Scanning and transmission electron microscopy allowed characterization of sinusoidal microtubular projections (700 nm average size, 200 µm average length, with bulging mitochondria and actin microfilaments); short, uniform, variously shaped nanotubular projections (100 nm, bidirectional communication); and tubule fragments (50 nm). This is the first study demonstrating that MSCs from human arteries constitutively interact through an articulate and dynamic tubule network allowing long-range cell to cell communication.
MATERIALS
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Carbon, mesoporous, nanopowder, graphitized, less than 250 ppm Al, Ti, Fe, Ni, Cu, and Zn combined
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Carbon, mesoporous, nanopowder, less than 500 ppm Al, Ti, Fe, Ni, Cu, and Zn combined
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Activated Charcoal Norit®, Norit® SX ultra, from peat, corresponds U.S. Food chemicals codex (3rd Ed.), steam activated and acid washed, highly purified, powder
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Activated Charcoal Norit®, Norit® GAC 1240W, from coal, for potable water processing, steam activated, granular
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Activated Charcoal Norit®, Norit® SX2, powder, from peat, multi-purpose activated charcoal, steam activated and acid washed
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Ethyl alcohol, Pure, 190 proof, ACS reagent, meets USP testing specifications, Excise Tax-free, Permit for use required
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Ethanol, BioUltra, for molecular biology, ≥99.8%, (absolute alcohol, without additive, A15 o1)
Carbon - Vitreous, foam, 150x150mm, 0.05g.cmué, porosity 96.5%, 24 pores/cm
Carbon - Vitreous, foil, 10x10mm, thickness 4.0mm, glassy carbon
Carbon - Vitreous, rod, 100mm, diameter 1.0mm, glassy carbon
Carbon - Vitreous, rod, 200mm, diameter 1.0mm, glassy carbon
Carbon - Vitreous, foil, 8x8mm, thickness 0.5mm, glassy carbon
Carbon - Vitreous, rod, 200mm, diameter 10mm, glassy carbon
Carbon - Vitreous, foil, 25x25mm, thickness 0.5mm, glassy carbon
Carbon - Vitreous, foil, 50x50mm, thickness 4.0mm, glassy carbon
Carbon - Vitreous, foam, 300x300mm, thickness 20mm, bulk density 0.05g/cm3, porosity 96.5%
Carbon - Vitreous, rod, 200mm, diameter 3.0mm, glassy carbon
Carbon - Vitreous, tube, 100mm, outside diameter 10mm, inside diameter 3mm, wall thickness 3.5mm, glassy carbon
Carbon - Vitreous, foil, 25x25mm, thickness 4.0mm, glassy carbon
Carbon - Vitreous, rod, 100mm, diameter 5.0mm, glassy carbon