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Merck

Verification of a canine PSMA (FolH1) antibody.

Anticancer research (2015-01-01)
Siegfried Wagner, Denise Maibaum, Andreas Pich, Ingo Nolte, Hugo Murua Escobar
ABSTRACT

Canine prostate cancer (PC) is a highly aggressive malignancy. However, in contrast to man, neither standard screening strategies nor curative therapeutic options exist for the companion animal. A prostate-specific membrane antigen (PSMA) screening as molecular marker akin to human PC is currently not available for dogs as data on specific canine PSMA detection are contradictory. To evaluate an antibody for specific canine PSMA detection by western blotting (WB), lysates of three canine prostatic cell lines (CT1258, DT08/40, DT08/46) were comparatively analyzed by WB and mass spectrometry (MS) to the human cell lines VCaP, LnCaP and PC-3. MS analyses of the detected canine proteins confirmed cross reactivity of the antibody clone YPSMA-1 with canine PSMA. The MS analyses of the extracted canine protein bands proved that the YPSMA-1 clone is as well specific for canine PSMA in WB and, thus, represents a reliable tool for comparative PSMA studies.

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SAFC
Sodium chloride solution, 5 M
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Sodium chloride solution, 0.85%
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4-tert-Octylphenol monoethoxylate solution, 10 μg/mL in acetone, analytical standard
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Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)
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Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
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Nitrotetrazolium Blue chloride, powder, electrophoresis grade
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Sodium bicarbonate, BioXtra, 99.5-100.5%
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Sodium chloride, BioXtra, ≥99.5% (AT)
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Sodium chloride, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
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Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
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Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
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Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
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Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
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Sodium dodecyl sulfate, ≥98.0% (GC)
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Sodium dodecyl sulfate, ≥90% ((Assay))
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Sodium dodecyl sulfate, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate
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Sodium dodecyl sulfate, ReagentPlus®, ≥98.5% (GC)
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Acrylamide, suitable for electrophoresis, ≥99% (HPLC), powder
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Nitrotetrazolium Blue chloride, ≥90.0% (HPLC)
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Sodium chloride solution, 5 M
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1 Estimated to ship on March 07, 2025
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MXP 17,315.00
5 kg
In Stock
Details...
MXP 64,563.00
25 kg
1 Estimated to ship on March 07, 2025
50 kg
1 Estimated to ship on March 07, 2025
Sigma-Aldrich
Sodium dodecyl sulfate, ≥98.0% (GC)
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SKUPack SizeAvailabilityPriceQuantity
25 g
In Stock
Details...
MXP 1,252.00
100 g
In Stock
Details...
This SKU is also available as equivalent grade in our Exclusive Redi-Dri™ free-flowing product line.
MXP 2,535.00
250 g
In Stock
Details...
MXP 5,685.00
500 g
In Stock
Details...
MXP 10,655.00
1 kg
1 Estimated to ship on March 07, 2025
This SKU is also available as equivalent grade in our Exclusive Redi-Dri™ free-flowing product line.
MXP 17,315.00
5 kg
In Stock
Details...
MXP 64,563.00
25 kg
1 Estimated to ship on March 07, 2025
50 kg
1 Estimated to ship on March 07, 2025