Application of the Fenton process in the elimination of helminth eggs.

Application of the Fenton process in the elimination of helminth eggs.

Journal of water and health (2014-12-05)

Sandra Isabel Escobar-Megchún, Hugo Alejandro Nájera-Aguilar, Magaly González-Hilerio, Javier Gutiérrez-Jiménez, Rubén Fernando Gutiérrez-Hernández, María Neftalí Rojas-Valencia

PMID25473981

ABSTRACT

This study relates to a method for evaluating the degradation efficiency of Ascaris lumbricoides eggs through a Fenton reaction, using hydrogen peroxide (H2O2) at 100, 250, and 500 mg/L, ferrous sulfate (FeSO4) at 458 mg/L Fe(2+) and pH values of 3, 4.5, and 6. The experiments were conducted according to a 3(2) experimental design, with 1:1, 3:1, and 5:1 (H2O2/Fe(2+)) molar ratios. The oxidation and flocculation stages were performed at 130 rpm during 2 hours and at 25-30 rpm during 20 min, respectively. As a result of the Fenton reaction, an average of 91.2% Ascaris lumbricoides egg degradation was achieved at pH 6 using a 500 mg/L dose of H2O2 and a 3:1 (H2O2/Fe(2+)) molar ratio. Thus, this process is an alternative for eliminating parasites that are resistant to conventional disinfection processes and significantly reduces the human health hazard they represent.

MATERIALS

Product Number

Brand

Product Description

88597

Millipore

Hydrogen peroxide solution, 3%, suitable for microbiology

44890

Sigma-Aldrich

Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)

IRMM524B

Iron, IRMM^®, certified reference material, 0.5 mm wire

356808

Sigma-Aldrich

Iron, foil, thickness 0.1 mm, ≥99.9% trace metals basis

267945

Sigma-Aldrich

Iron, chips, 99.98% trace metals basis

F8263

Sigma-Aldrich

Iron(II) sulfate heptahydrate, suitable for plant cell culture, ≥99%

F8633

Sigma-Aldrich

Iron(II) sulfate heptahydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

215422

Sigma-Aldrich

Iron(II) sulfate heptahydrate, ACS reagent, ≥99.0%

GF35663935

Iron, wire reel, 5m, diameter 0.025mm, as drawn, 99.99+%

GF05179709

Iron, wire reel, 1m, diameter 0.5mm, as drawn, 99.998%

GF02717502

Iron, wire reel, 0.5m, diameter 0.25mm, hard, 99.99+%

GF47408430

Iron, wire reel, 0.5m, diameter 1.0mm, as drawn, 99.99+%

GF61040023

Iron, wire reel, 5m, diameter 0.25mm, hard, 99.99+%

GF96915134

Iron, wire reel, 50m, diameter 0.5mm, as drawn, 99.95%

GF81282135

Iron, wire reel, 2m, diameter 0.25mm, annealed, 99.99+%

GF82602176

Iron, wire reel, 0.05m, diameter 0.5mm, as drawn, 99.998%

GF08606606

Iron, rod, 500mm, diameter 12.7mm, as drawn, 99.95%

GF29558582

Iron, foil, 50x50mm, thickness 2mm, as rolled, 99.99+%

GF17042082

Iron, tube, 200mm, outside diameter 4.0mm, inside diameter 3.6mm, wall thickness 0.2mm, as drawn, 99.5%

GF62438520

Iron, rod, 200mm, diameter 25mm, as drawn, 98+%

GF61545674

Iron, tube, 100mm, outside diameter 8.0mm, inside diameter 5mm, wall thickness 1.5mm, annealed, 99.5%

GF85519299

Iron, foil, 300x300mm, thickness 1.0mm, as rolled, 99.5%

GF03803587

Iron, foil, 300x300mm, thickness 2.0mm, as rolled, armco« soft ingot 99.8%

GF03053001

Iron, wire reel, 1m, diameter 0.025mm, as drawn, 99.99+%

GF29986981

Iron, foil, 300x300mm, thickness 0.15mm, hard, 99.5%

GF69332519

Iron, foil, 50x50mm, thickness 0.25mm, as rolled, 99.99+%

GF02617657

Iron, wire reel, 1000m, diameter 0.125mm, hard, 99.5%

GF84152156

Iron, foil, 10mm disks, thickness 0.005mm, 99.85%

GF40975601

Iron, tube, 500mm, outside diameter 3.18mm, inside diameter 1.96mm, wall thickness 0.61mm, as drawn, 99.5%

GF06733818

Iron, tube, 200mm, outside diameter 5.0mm, inside diameter 4.5mm, wall thickness 0.25mm, as drawn, 99.5%