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  • Biochemical characterization of IMP-30, a metallo-β-lactamase with enhanced activity toward ceftazidime.

Biochemical characterization of IMP-30, a metallo-β-lactamase with enhanced activity toward ceftazidime.

Antimicrobial agents and chemotherapy (2013-07-10)
Kevin M Pegg, Eleanor M Liu, Alecander E Lacuran, Peter Oelschlaeger
ABSTRACT

IMP-type enzymes constitute a clinically important family of metallo-β-lactamases that has grown dramatically in the past decade to its current 45 known members. Here, we report the biochemical characterization of IMP-30 in comparison to IMP-1, from which it deviates by a single E59K mutation. Kinetics, MIC assays, docking, and molecular dynamics simulations support a scenario in which Lys59 interacts with the ceftazidime R1 group, resulting in increased water access and enhanced turnover and MIC of ceftazidime.

MATERIALS
Product Number
Brand
Product Description

Ceftazidime, European Pharmacopoeia (EP) Reference Standard
Ceftazidime for peak identification, European Pharmacopoeia (EP) Reference Standard