- ChIP for identification of p53 responsive DNA promoters.
ChIP for identification of p53 responsive DNA promoters.
Methods in molecular biology (Clifton, N.J.) (2012-11-15)
Jun-Ming Liao, Hua Lu
PMID23150449
ABSTRACT
Chromatin immunoprecipitation assay (ChIP) has been frequently used to determine whether a transcriptional regulator can bind to a specific DNA element in the chromatin content of cells. Here, we describe a detailed protocol for this assay with hands-on tips based on our own experience in working on the transcriptional regulator and tumor suppressor p53.
MATERIALS
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Sigma-Aldrich
Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)