ZRB1113
Anti-LILRB4 Antibody, clone 1H12 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1H12, monoclonal
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt ~49.36 kDa
observed mol wt ~50 kDa
species reactivity
human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1H12 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects human Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4). It targets an epitope within 19 amino acids from the extracellular domain.
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the extracellular domain of human Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4).
Application
Quality Control Testing
Evaluated by Western Blotting in MDA-MB-435 lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LILRB4 in MDA-MB-435 cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LILRB4 in NK-92 cells.
Affinity Binding Assay: A representative lot of this antibody bound LILRB4 peptide with a KD of 2.5 x 10-7 in an affinity binding assay.
Flow Cytometry Analysis: 1 μg from a representative lot detected LILRB4 in NK-92 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in MDA-MB-435 lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LILRB4 in MDA-MB-435 cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LILRB4 in NK-92 cells.
Affinity Binding Assay: A representative lot of this antibody bound LILRB4 peptide with a KD of 2.5 x 10-7 in an affinity binding assay.
Flow Cytometry Analysis: 1 μg from a representative lot detected LILRB4 in NK-92 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-LILRB4, clone 1H12 ZooMAb®, Cat. No. ZRB1113, is a rabbit recombinant monoclonal antibody that specifically targets LILRB4 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting.
Target description
Leukocyte immunoglobulin-like receptor subfamily B member 4 (UniProt: Q8NHJ6; also known as CD85 antigen-like family member K, Immunoglobulin-like transcript 3, ILT-3, Leukocyte immunoglobulin-like receptor 5, LIR-5, Monocyte inhibitory receptor HM18, CD85k, LILRB4) is encoded by the LILRB4 (Gene ID: ILT3, LIR5) gene (Gene ID: 11006) in human. LILRB4 is a single-pass type I membrane protein that serves as a receptor for class I MHC antigens and recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. It is involved in the downregulation of the immune response and the development of tolerance, such as towards transplants. It is reported to inhibit receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. LILRB4 expression has been reported in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells and is induced upon contact with CD8+ CD28- alloantigen-specific T suppressor (Ts) cells. LILRB4 is also a tumor-associated antigen that is highly expressed on monocytic AML cells where its expression is more specific and can be used to differentiate monocytic AML (M5) from AML M1-M3. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 22-259), a transmembrane domain (aa 260-280), and a cytoplasmic domain (aa 181-448). It contains three copies of immunoreceptor tyrosine-based inhibitor motif (ITIM) that are involved in modulation of cellular responses and the phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: John, S. et al. (2018). Mol. Therap. 26(10); 2487-2495).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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