SEQXE
SeqPlex DNA Amplification Kit
For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.
Synonym(s):
SeqPlex Enhanced DNA Amplification Kit, WGA kit, whole genome amplification kit
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About This Item
UNSPSC Code:
41121800
NACRES:
NA.55
Recommended Products
technique(s)
whole genome amplification: suitable
shipped in
wet ice
storage temp.
−20°C
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General description
SeqPlex™ Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful next generation sequencing library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow. This kit is an extension of the WGA product line and has been developed to integrate into the Illumina®, SOLiD™, or 454 sequencing workflows.
Application
SeqPlex™ DNA Amplification Kit has been used for whole genome amplification.
Features and Benefits
- Random priming technology amplifies fragmented DNA such as ChIP or FFPE
- Facilitates sequencing from as little as 100 pg of ChIP DNA
- Enhanced primers for complete genome coverage, minimal sequence bias, primer removal, and amplicon size ideal for next gen sequencing
- Compatible with Illumina®, SOLiD™, or 454 library prep for next generation sequencing
Other Notes
SEQXE-500RXN is manufactured on-demand. Contact technical services at techserv@sial.com for more information.
Legal Information
Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
iCAT is a registered trademark of University of Washington
Kit Components Also Available Separately
Product No.
Description
SDS
- W4502Water, Nuclease-Free Water, for Molecular BiologySDS
- Library Preparation Buffer
recommended
Product No.
Description
Pricing
required but not provided
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Protocols
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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