D3404

Plasmid DNA from E. coli RRI

pUC19, buffered aqueous solution

• CAS Number: 9007-49-2
• MDL number: MFCD00166896
• UNSPSC Code: 41105600
• NACRES: NA.85

Properties

• grade: for molecular biology
• form: buffered aqueous solution
• shipped in: dry ice
• storage temp.: −20°C
• InChI: 1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)
• InChI key: AWBASQCACWFTGD-UHFFFAOYSA-N

Description

General description

The pUC19 plasmid (2,686 bp) confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the B-galactosidase gene; the plasmid has thirteen unique sites in the MCS (Acc I, BamH I, EcoR I, Hinc II, Hind III, Kpn I, Pst I, Sac I, Sal I, Sma I, Sph I, Xba I, and Xma I).

Application

Plasmid DNA from Escherichia coli RRI has been used for imaging of DNA (nanostructure) via atomic force microscopy.

Biochem/physiol Actions

These plasmids confer ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene; pUC8 and 9 have nine unique sites within the MCS while pUC18 and 19 have thirteen.
Foreign DNA inserted at the MCS abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium. The orientations of the MCS regions in the pUC plasmids are analogous to those of the corresponding M13 phage.

Components

pUC19 Plasmid DNA, 10 μg is supplied at approximately 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Principle

Foreign DNA inserted at the MCS interrupts the β-galactosidase gene and abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable