A5533

Alizarin Red S

certified by the Biological Stain Commission, powder

• Synonym(s): 3,4-Dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt, Alizarin Carmine, Alizarin Red, water soluble, Alizarin sodium monosulfonate, Alizarin sulfonate sodium, Alizarinsulfonic acid sodium salt, Sodium alizarinsulfonate
• Empirical Formula (Hill Notation): C₁₄H₇NaO₇S
• CAS Number: 130-22-3
• Molecular Weight: 342.26
• Colour Index Number: 58005
• Beilstein: 4117090
• EC Number: 204-981-8
• MDL number: MFCD00013049
• UNSPSC Code: 12171500
• PubChem Substance ID: 24890988
• NACRES: NA.47

Properties

• Product Name: Alizarin Red S, certified by the Biological Stain Commission
• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• concentration: 0.015 g/L in 0.1 M NaOH
• technique(s): titration: suitable
• color: dark red; yellow-orange to dark orange
• solubility: H₂O: 1 mg/mL
• ε (extinction coefficient): 592-598 nm at 0.015 g/L in 0.1 M NaOH
• application(s): diagnostic assay manufacturing; hematology; histology

Description

Application

• Alizarin Red S has been used for checking the calcium deposits in osteogenic culture.[1]
• It has been used for the quantitative analyses of calcification in aortic explants.[2]
• It has been used for mineral staining in osteoblast cultures.[3][4]

Biochem/physiol Actions

Alizarin Red S is used for the staining of cartilage and bone.[5] It reacts with calcium, thereby helping in the diagnosis of calcium deposits.[6] It is also used to study carbohydrate–boronic acid interactions.[7]

Suitability

Certified for use in Dahl′s method for calcium

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves