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antibody form
purified antibody
clone
4
monoclonal
purified by
using protein G
species reactivity
mouse, virus
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
UniProt accession no.
General description
Recombinant 3D polymerase from Enterovirus 71.
Specificity
Clone 4 is a mouse monoclonal antibody that specifically detects Enterovirus 71 3D polymerase in EV71 infected cells.
Application
Immunofluorescence Analysis: A representative lot detected Enterovirus 71 3D in Immunofluorescnece applications (Huang, H.I., et. al. (2014). Virology. 468-470:592-600; Huang, P.N., et. al. (2017). PLoS Pathog. 13(5): e1006375).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Enterovirus 71 3D in Immunoprecipitation applications (Huang, P.N., et. al. (2017). PLoS Pathog. 13(5): e1006375).
Western Blotting Analysis: A representative lot detected Enterovirus 71 3D in Western Blotting applications (Huang, H.I., et. al. (2014). Virology. 468-470:592-600; Huang, H.I., et. al. (2018). PLoS One. 13(1): e0191617).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Enterovirus 71 3D in Immunoprecipitation applications (Huang, P.N., et. al. (2017). PLoS Pathog. 13(5): e1006375).
Western Blotting Analysis: A representative lot detected Enterovirus 71 3D in Western Blotting applications (Huang, H.I., et. al. (2014). Virology. 468-470:592-600; Huang, H.I., et. al. (2018). PLoS One. 13(1): e0191617).
Quality
Evaluated by Western Blotting in Enterovirus 71 infected human embryonal rhabdomyosarcoma cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected Enterovirus 71 3D in Enterovirus 71 infected human embryonal rhabdomyosarcoma cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected Enterovirus 71 3D in Enterovirus 71 infected human embryonal rhabdomyosarcoma cell lysates.
Physical properties
~52 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Virus (Enterovirus)
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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