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B4875

Sigma-Aldrich

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride

trypsin substrate, chromogenic, elastase substrate, ≥98%, powder

Synonym(s):

Nα-Benzoyl-DL-arginine p-nitroanilide hydrochloride, BANI, BAPNA

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100 MG
R$504.00
500 MG
R$598.00
1 G
R$727.00
5 G
R$2,221.00
10 G
R$3,812.00

R$504.00


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100 MG
R$504.00
500 MG
R$598.00
1 G
R$727.00
5 G
R$2,221.00
10 G
R$3,812.00

About This Item

Empirical Formula (Hill Notation):
C19H22N6O4 · HCl
CAS Number:
Molecular Weight:
434.88
Beilstein:
4081878
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

R$504.00


In StockDetails


Request a Bulk Order

Product Name

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, ≥98%

Quality Level

Assay

≥98%

form

powder

color

, white to light yellow to light brown

solubility

DMSO: 50 mg/mL (colorless to dark green-yellow to dark brown-yellow)

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)NCCCC(NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H

InChI key

DEOKFPFLXFNAON-UHFFFAOYSA-N

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General description

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride (DL-BAPNA) is a chromogenic substrate for proteolytic enzymes such as trypsin, amidase, and balterobin. Hydrolysis of DL-BAPNA at the bond between the arginine and the p-nitroaniline moieties releases the chromophore p-nitroaniline, which can be detected by colorimetric analysis.

Application

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride has been used as a substrate for trypsin[1] and elastase in enzyme analysis.[2]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Substrates

A chromogenic trypsin substrate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Tooba Siddiqui et al.
Journal of fluorescence, 29(4), 969-979 (2019-07-22)
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Biological trace element research, 149(2), 190-196 (2012-04-28)
One hundred-eighty piglets (Duroc × Landrace × Yorkshire), with an average initial weight of 7.4 kg weaned at 27 ± 1 days of age, were used to evaluate the effects of dietary zinc oxide-montmorillonite hybrid (ZnO-MMT) on growth performance, diarrhea
Digestive enzymes and stylet morphology of Deraeocoris nigritulus (Uhler)(Hemiptera: Miridae) reflect adaptations for predatory habits
Boyd DW
Annals of the Entomological Society of America, 96(5), 667-671 (2014)
Nguyen Van Quan et al.
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 27(5), 643-649 (2019-07-13)
This study was the first to detect the presence of the two compounds momilactone A (MA) and momilactone B (MB) in rice bran using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). By in vitro assays, both MA and MB exhibited potent inhibitory
Carola Engler et al.
PloS one, 4(5), e5553-e5553 (2009-05-14)
We have developed a protocol to assemble in one step and one tube at least nine separate DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained containing the desired construct. This protocol is based on the

Protocols

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

Questions

  1. Why do the bottoms of the vials/bottles of product B4875-100MG (Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride) appear to have no bottom? How is the product contained within the vials despite this unusual appearance? Is this a packaging defect?

    1 answer
    1. The product is packaged in bottomless glass bottles with an inserted fused cone. The micro-vials contain the product within an inner cone, while the bottomless outer glass cylinder, serves as a stand. This design is intentional and not a defect.

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