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Merck

One-flask synthesis of cyclic diguanosine monophosphate (c-di-GMP).

Current protocols in nucleic acid chemistry (2012-03-08)
Barbara L Gaffney, Roger A Jones
ABSTRACT

The bacterial signaling molecule, cyclic diguanosine monophosphate (c-di-GMP), plays a key role in controlling biofilm formation and pathogenic virulence, among many other functions. It has widespread consequences for human health, and current research is actively exploring its molecular mechanisms. The convenient one-flask, gram-scale synthesis of c-di-GMP described here will facilitate these efforts.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Chloro-5,5-dimethyl-1,3,2-dioxaphosphorinane 2-oxide, 95%
Sigma-Aldrich
Triethylamine, BioUltra, ≥99.5% (GC)
Sigma-Aldrich
Triethylamine, for protein sequence analysis, ampule, ≥99.5% (GC)
Sigma-Aldrich
Triethylamine, SAJ first grade, ≥98.0%
Sigma-Aldrich
Triethylamine, for amino acid analysis, ≥99.5% (GC)
Sigma-Aldrich
Triethylamine, puriss. p.a., ≥99.5% (GC)
Sigma-Aldrich
Triethylamine, ≥99.5%
Sigma-Aldrich
Triethylamine, ≥99%
Sigma-Aldrich
Triethylamine, ≥99.5%
Sigma-Aldrich
Triethylamine hydrochloride, ≥99.0% (AT)