ZRB1724
Anti-Jagged1 Antibody, clone 1C23 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Protein jagged-1;hJ1;CD339
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1C23, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 133.8 kDa
observed mol wt ~17 kDa
purified by
using Protein A
species reactivity
human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
epitope sequence
C-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... JAG1(182)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1C23 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Jagged 1. It targets an epitope within 24 amino acids from the C-terminal, cytoplasmic domain.
Immunogen
KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal, cytoplasmic domain of human Jagged-1.
Application
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Jagged1 in human kidney tissue sections.
Tested applications
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Jagged1 in one million Jurkat cells.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Jagged1 in HT-29 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Jagged1 in HepG2 cells.
Affinity Binding Assay: A representative lot of this antibody bound Jagged1 with a KD of 1.1 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Jagged1 in human kidney tissue sections.
Tested applications
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Jagged1 in one million Jurkat cells.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Jagged1 in HT-29 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Jagged1 in HepG2 cells.
Affinity Binding Assay: A representative lot of this antibody bound Jagged1 with a KD of 1.1 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Protein jagged-1 (UniProt: P78504; also known as Jagged1, hJ1, CD339) is encoded by the JAG1 (also known as JAGL1) gene (Gene ID: 182) in human. Jagged 1 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to generate the mature form that contains a large extracellular domain (aa 34-1067), a transmembrane domain (aa 1068-1093), and a cytoplasmic domain (aa 1094-1218). Jagged 1 is widely expressed in adult and fetal tissues. Its expression is observed in 32-52 days old embryos in the distal cardiac outflow tract and in pulmonary artery, pancreas, mesocardium, neural tube, and around the major bronchial branches. It serves as ligand for multiple Notch receptors and is involved in mediation of Notch signaling and cell-fate decisions during hematopoiesis. It is also reported to enhance FGF-induced angiogenesis. Its binding and activating ability are tightly regulated by Notch receptor glycosylation. Jagged 1 also plays roles in various aspects of cancer biology, including tumor angiogenesis, neoplastic cell growth, metastasis, epithelial-mesenchymal transition, and resistance to therapy. Its expression in other cells of tumor microenvironment has also been described. Jagged 1 expression can also be induced by other signaling pathways that are important in tumor growth and metastasis, including TGF-β, WNT/β-catenin, IL-6, and NF-kB. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, D., et al. (2014). Front. Oncol. 4; 254; Cordle, J., et al. (2008). Nat. Struct. Mol. Biol. 15(8); 849-857).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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