SRP0106

HDAC-5 human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Synonym(s): HD5, Histone deacetylase 5, NY-CO-9
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥80% (SDS-PAGE)
• form: aqueous solution
• potency: ≥1 units/mg
• mol wt: 51 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_001015053
• UniProt accession no.: Q9UQL6
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... HDAC5(10014)

Description

General description

Human HDAC5, catalytic domain, GenBank Accession No. NM_001015053, amino acid 657-1123 with C-terminal His tag, MW = 51 kDa, expressed in baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable