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Key Documents

SAB5600096

Sigma-Aldrich

Anti-His-Tag Chimeric antibody, Human Monoclonal

recombinant, expressed in HEK 293 cells, clone RMH01, purified immunoglobulin

Synonym(s):

Anti-His tag antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

recombinant

expressed in HEK 293 cells

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RMH01, monoclonal
recombinant monoclonal

form

buffered aqueous glycerol solution

technique(s)

capture ELISA: 0.1-2 μg/mL
western blot: 0.1-1 μg/mL

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Hexahistidine tag (His-tag) consists of four to six histidines fused genetically. It is widely used to purify proteins by immobilized metal-ion affinity chromatography (IMAC).

Specificity

This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.

Immunogen

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jonathan F Lovell et al.
BMC medicine, 20(1), 462-462 (2022-11-30)
Numerous vaccine strategies are being advanced to control SARS-CoV-2, the cause of the COVID-19 pandemic. EuCorVac-19 (ECV19) is a recombinant protein nanoparticle vaccine that displays the SARS-CoV-2 receptor-binding domain (RBD) on immunogenic nanoliposomes. Initial study of a phase 2 randomized
Tandem immobilized metal-ion affinity chromatography/immunoaffinity purification of His-tagged proteins?evaluation of two anti-His-tag monoclonal antibodies
Muller KM, et al.
Analytical Biochemistry, 259(1), 54-61 (1998)
Loree Heller et al.
Journal of cellular and molecular medicine, 24(15), 8772-8778 (2020-07-02)
In neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, multiple sclerosis and amyotrophic lateral sclerosis, neuroinflammation can lead to blood-brain barrier (BBB) breakdown. After intravenous or intra-arterial injection into mice, endothelial progenitor cells (EPCs) home to the damaged BBB to
Reynald Thinard et al.
Pharmaceutics, 14(7) (2022-07-28)
The failures of anti-β-amyloid immunotherapies suggested that the very low fraction of injected antibodies reaching the brain parenchyma due to the filtering effect of the BBB may be a reason for the lack of therapeutic effect. However, there is no

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