Name: MISSION<SUP>®</SUP> miRNA Product Offerings
Brand: SIGMA

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<p><b>Synthetic miRNA Inhibitors (S-TuD) Negative Controls</b></p>


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<h2><br>
N-TER<sup>™</sup> Nanoparticle siRNA Transfection System</h2>


<h2>Lentiviral Library- Request A quote</h2>


<ul>
<li>Peptide transfection reagent for use in difficult to transfect cells</li>
</ul>


<h4>Lentiviral and Synthetic miRNA Negative Controls Measure transduction efficiency or monitor off-target effects.</h4>
<ul>
<li>All miRNA mimic and inhibitor negative controls are carefully selected, and have no known homology to known human or mouse gene sequences</li>
<li>Each negative control contains either a <i>Arabidopsis thaliana</i> sequence (Control 1) or a <i>Caenorhabditis elegans</i> sequence (Control 2)</li>
<li>Lenti TuD Negative controls available: <a href="/product/sigma/HLTUD001C">HLTUD001C</a> and <a href="/product/sigma/HLTUD002C">HLTUD002C</a></li>
<li>Lenti miR Negative Controls available: <a href="/product/sigma/NCLMIR001">NCLMIR001</a> and <a href="/product/sigma/NCLMIR002">NCLMIR002</a></li>
<li>Synthetic miRNA mimics Negative Controls available as <a href="/product/sigma/HMC0002">HMC0002</a> and <a href="/product/sigma/HMC0003">HMC0003</a></li>
<li>Synthetic miRNA Inhibitors Negative Controls available as <a href="/product/sigma/NCSTUD001">NCSTUD001</a> and <a href="/product/sigma/NCSTUD002">NCSTUD002</a></li>
</ul>


<h4>Bench-top transcriptome-wide human miRNA and ncRNA gene target identification.</h4>
<ul>
<li>Convenience: Perform dual screening with one vector due to the innovative dual selection cassette</li>
<li>Simplicity: 3 easy steps: Library Transfection, microRNA Transfection and Ganciclovir Selection, and PCR and Sequencing.</li>
<li>Relevance: The only method for genome-wide functional identification of miRNA targets</li>
<li>Custom miRNA mimic synthesis capabilities: custom species sequences, quantities, and modifications</li>
</ul>


<h4>Most comprehensible list of miRNA mimics</h4>
<ul>
<li>Designed using a proprietary algorithm based upon the Tough Decoy (TuD) design</li>
<li>Long-lasting inhibition at very low nM concentrations</li>
<li>Excellent resistance to cellular nucleases</li>
<li>Structure provides potent inhibition of the desired miRNA</li>
<li>Custom synthesis capabilities: custom species sequences, quantities, and modifications</li>
</ul>


<h4>Choose from over 3,000+ human and mouse miRNA inhibitors</h4>
<ul>
<li>Designed using a proprietary algorithm based upon the Tough Decoy (TuD) design</li>
<li>Allows for potent inhibition of the desired miRNA</li>
<li>Stable, long-term expression of miRNA</li>
<li>Efficient delivery of mimic into wide variety of cell types</li>
<li>Species include both human and mouse: 1,953 human lenti miRNA inhibitors and 1,214 mouse lenti miRNA inhibitors</li>
<li>Custom options available</li>
</ul>


<h4>Different gene pathway of interest? Want a more complete list?</h4>


<h4>Choose from over 2500+ miRNA mimics</h4>
<ul>
<li>Most comprehensive collection of miRNA human mimics available (mirBase ver. 21)</li>
<li>Available as a complete library or individual miRNA mimics</li>
<li>Stable, long-term expression of miRNA</li>
<li>Efficient delivery of mimic into wide variety of cell types</li>
<li>The ability to select for cells that express the miRNA of interest</li>
<li>Custom options available</li>
</ul>


<ul>
<li>Gene family sets are fully customizable allowing for complete flexibility to add or delete clone or gene content.</li>
</ul>


<h4>Validate miRNA targets and study post-transcriptional gene regulation with lentiviral 3′UTR reporters.</h4>
<ul>
<li>A genome-wide collection of pre-cloned reporter constructs for over 10,000 human 3’UTRs</li>
<li>Constructs are composed of a human 3’UTR fused to a novel luciferase reporter</li>
<li>Relevance: The only method for genome-wide functional identification of miRNA targets</li>
<li>Optimized Renilla luciferase delivers higher activity</li>
<li>System utilizes a lentiviral vector for delivery into difficult to transfect cell lines, including differentiated and non-dividing cells</li>
<li>Superb virus quality delivers superior performance</li>
<li>Negative controls, miRNA Mimics, and MISSION LightSwitch Luciferase Assay Reagent<sup>™</sup> are available to complete the validation workflow.</li>
</ul>


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MISSION® miRNA Product Offerings; Synonyms: Custom miRNA,Human miRNA cDNA Library,Lenti TuDs,Lentiviral miRNA mimics,MISSION® 3′UTR Lenti GoClone,MISSION® Lentiviral miRNA inhibitors,MISSION® LightSwitch Luciferase Assay Reagent,MISSION® Target ID Library,MISSION® miRNA Human inhibitors,MISSION® miRNA Human mimics,MISSION® miRNA Workflow,Synthetic TuDs,Synthetic miRNA inhibitors,Synthetic miRNA mimics,Tough Decoy Design,miRBase version 21,miRNA Identification,miRNA Library,miRNA Screening,miRNA Validation at Sigma-Aldrich

MISSION® miRNA Product Offerings | Sigma-Aldrich

MISSION<SUP>®</SUP> miRNA Product Offerings

Synonym: Custom miRNA, Human miRNA cDNA Library, Lenti TuDs, Lentiviral miRNA mimics, MISSION® 3′UTR Lenti GoClone, MISSION® Lentiviral miRNA inhibitors, MISSION® LightSwitch Luciferase Assay Reagent, MISSION® Target ID Library, MISSION® miRNA Human inhibitors, MISSION® miRNA Human mimics, MISSION® miRNA Workflow, Synthetic TuDs, Synthetic miRNA inhibitors, Synthetic miRNA mimics, Tough Decoy Design, miRBase version 21, miRNA Identification, miRNA Library, miRNA Screening, miRNA Validation

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