11742027001

α-Glucosidase Assay

suitable for activity assay, kit of 1 (5 components)

• Synonym(s): α-Glucosidase, Alpha-glucosidase activity assay kit, Maltase, glucosidase, glycosidase, neutral alpha-glucosidase (NAG)
• UNSPSC Code: 12161503

Properties

• usage: sufficient for 30 tests (and 10 blanks)
• species reactivity: human
• packaging: kit of 1 (5 components)
• manufacturer/tradename: Roche
• storage condition: (Keep container tightly closed in a dry and well-ventilated place.)
• technique(s): activity assay: suitable
• detection method: colorimetric
• storage temp.: 2-8°C

Description

General description

Colorimetric assay for the quantitative determination of α-glucosidase (maltase) in human semen research samples. α-Glucosidase is a key enzyme involved in carbohydrate hydrolysis. It is essential for the conversion of oligosaccharides and disaccharides into glucose.

Application

α-Glucosidase Assay Kit has been used to measure neutral α-glucosidase (NAG) activity in seminal samples for the evaluation of epididymal function.

Features and Benefits

• Fast and sensitive: The analysis can be performed using only 15 μl of semen or seminal plasma, ensuring accurate results while minimizing the sample volume needed.
• Specific: The assay is designed to measure the activity of neutral α-glucosidase in human semen samples in life science research applications. It is not designed for diagnostic applications and cannot be used to measure α-glucosidase in other specimens.
• Compatible: The assay is compatible with microplate assay formats and standard cuvette formats

Packaging

1 kit containing 5 components.

Components

The α-Glucosidase Assay contains the substrate and all buffers for the measurement of α-glucosidase in human semen or seminal plasma samples. It also contains a standard substance to set up a standard curve.

Preparation Note

Storage conditions (working solution): For stability of reconstituted kit components see section "General considerations".

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

• Reaction Buffer
• Substrate Solution containing 4-nitrophenyl-α-D-glucopyranoside
• Stopping Buffer
• Castanospermine
• Standard Solution containing 4-nitrophenol

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): does not flash
• Flash Point(C): does not flash