DR1052

Anti-SALL4 Mouse mAb (6E3)

liquid, clone 6E3, Calbiochem^®

• Synonym(s): Anti-SAL-Like 4
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 6E3, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SALL4(57167) ; mouse ... Sall4(99377)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~100 kDa SALL4 protein.

Recognizes the ~100 kDa SALL4 protein in HeLa and NIH3T3 cells and human testes tissue.

This Anti-SALL4 Mouse mAb (6E3) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of SALL4.

Immunogen

A recombinant polypeptide corresponding to amino acids 954-1054 of human SALL4, expressed as a GST fusion protein

NA

Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (0.2 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HeLa cells, NIH3T3 cells, Human testes tissue

Other Notes

Cauffman, G., et al. 2009. Hum. Reprod.24 63.
Forte, A., et al. 2009. Mol. Med.15 392.

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable