Skip to Content
Merck
  • Multiresidue determination of estrogens in different dairy products by ultra-high-performance liquid chromatography triple quadrupole mass spectrometry.

Multiresidue determination of estrogens in different dairy products by ultra-high-performance liquid chromatography triple quadrupole mass spectrometry.

Journal of chromatography. A (2017-04-02)
Bárbara Socas-Rodríguez, Antonio V Herrera-Herrera, Javier Hernández-Borges, Miguel Ángel Rodríguez-Delgado
ABSTRACT

In this work, a simple and fast methodology has been validated and applied for the analysis of a group of 22 estrogenic compounds including eight phytoestrogens (i.e. daidzein, enterodiol, glycitein, enterolactone, genistein, formononetin, prunetin, biochanin A), six mycotoxins (β-zearalanol, β-zearalenol, α-zearalanol, α-zearalenol, zearalanone, zearalenone) as well as four synthetic (i.e. ethynylestradiol, diethylstilbestrol, dienestrol, hexestrol) and four natural estrogens (i.e. estriol, 17β-estradiol, 17α-estradiol, estrone) in different dairy products. Extraction was carried out using the QuEChERS method while separation, determination and quantification of the target analytes were achieved by ultra-high-performance liquid chromatography coupled to triple quadrupole mass spectrometry with an electrospray ionization interface. The methodology was validated for four dairy product samples with relevant interest for the population including skimmed and whole cheese and goat and cow kefir, using 17β-estradiol-2,4,16,16,17-d

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Enterodiol, ≥95.0% (HPLC)
Sigma-Aldrich
Octadecylsilane, 97%
Sigma-Aldrich
Prunetin, ≥98.0% (TLC)
Sigma-Aldrich
Enterolactone, ~95% (HPLC)
Sigma-Aldrich
Hexarelin, ≥90% (HPLC)
Supelco
(±)-Enterolactone, analytical standard