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50533-U

Supelco

Ascentis® Express 90 Å C18 (5 μm) HPLC Columns

L × I.D. 7.5 cm × 4.6 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Express C18, 5 μm HPLC Column, 5 μm particle size, L × I.D. 7.5 cm × 4.6 mm

material

stainless steel column

Agency

suitable for USP L1

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

7.5 cm × 4.6 mm

surface area

90 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

90 Å pore size

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

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General description

Ascentis® Express 5 μm C18 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core® particle design. The Fused-Core® particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5-micron thick porous shell and the highly uniform overall particle size of 5-microns. The densely bonded, extensively endcapped dimethyloctadecyl stationary phase of Ascentis Express 5 μm C18 provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Silvia Jakabová et al.
Journal of chromatography. A, 1232, 295-301 (2012-03-07)
Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a
Petra Šilarová et al.
Food chemistry, 237, 471-480 (2017-08-03)
The degradation of catechins and other phenolics in green tea infusions were monitored using fast HPLC/MS separation. The final separation was performed within 2.5min using Ascentis Express C18 column (50mm×2.1mm i.d.) packed with 2μm porous shell particles. Degradation was studied
Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within
Francesco Pio Prencipe et al.
Journal of chromatography. A, 1349, 50-59 (2014-05-27)
The study was aimed at developing a new analytical method for the metabolite fingerprinting of bioactive compounds in Humulus lupulus L. (hop), together with a simple extraction procedure. Different extraction techniques, including maceration, heat reflux extraction (HRE), ultrasound-assisted extraction (UAE)
Massimo Tacchini et al.
Journal of pharmaceutical and biomedical analysis, 146, 1-6 (2017-08-26)
The genus Echinacea (Asteraceae) includes species traditionally used in phytotherapy. Among them, Echinacea pallida (Nutt.) Nutt. root extracts are characterized by a representative antiproliferative activity, due to the presence of acetylenic compounds. In this study, supercritical fluid extraction (SFE) was

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