Skip to Content
Merck
All Photos(1)

Key Documents

X4875

Sigma-Aldrich

Xanthine Oxidase from bovine milk

Grade IV, ammonium sulfate suspension, ≥0.1 units/mg protein

Synonym(s):

XOD, Xanthine:oxygen oxidoreductase

Sign Into View Organizational & Contract Pricing


About This Item

CAS Number:
Enzyme Commission number:
1.17.3.2.
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bovine milk

type

Grade IV

form

ammonium sulfate suspension

specific activity

≥0.1 units/mg protein

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

Research area: Cell CycleXanthineoxidase (XO) is a homodimer. It is made up of two sub-units each made up of onemolybdenum and two iron-sulphur (2Fe-2S) centers.
Formerly E.C. 1.1.3.22

Application

Xanthine Oxidase from bovine milk has been used in superoxide dismutaseactivity assay.Xanthine oxidase may be suitable for producing amperometric biosensors and designing-flavonoid-type inhibitors against XO for treating inflammatory diseases, gout, and hyperuricemia. It may also be used in the synthesis of superoxide because of aldehyde oxidation.

Biochem/physiol Actions

Although xanthine oxidase is found widely distributed in multiple tissues, it is highly expressed in endothelial and epithelial cells. Its expression was also increased in infected tissues where it was shown to have an anti-microbial effect due to its ability to generate reactive oxygen species. Xanthineoxidase plays an important role in catalyzing the hydroxylation ofhypoxanthine to Xanthine. Later, xanthine is converted to uric acid isexcreted by the kidneys.
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Unit Definition

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Physical form

Suspension in 2.3 M (NH4)2SO4 containing 1 mM sodium salicylate

Analysis Note

Protein determined by biuret

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Role of xanthine oxidoreductase as an antimicrobial agent.
Hannah M Martin et al.
Infection and immunity, 72(9), 4933-4939 (2004-08-24)
Evolution of the anticholinesterase, antioxidant, and anti-inflammatory activity of Epilobium angustifolium L. infusion during in vitro digestion
Szwajgier D, et al.
Journal of functional foods, 85, 104645-104645 (2021)
Szymon Byczkiewicz et al.
Nutrients, 16(11) (2024-06-19)
In the present study, the effect of the addition of quince and collagen type I and III to dessert chocolate on its functional properties was determined. The study evaluated the antioxidant potential of the tested formulations using the FRAP method
M B Betancor et al.
Fish physiology and biochemistry, 41(3), 705-720 (2015-03-10)
In vertebrates, selenium (Se) is an essential micronutrient for vertebrates that is involved in antioxidant protection and thyroid hormone regulation among other roles and functions through its incorporation into proteins, the selenoproteins. Long-chain polyunsaturated fatty acids, such as docosahexaenoic acid
Xanthine oxidase: isolation, assays of activity, and inhibition
Kostic D, et al.
Journal of Chemistry, 2015(1), 294858-294858 (2015)

Articles

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service