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O3757

Sigma-Aldrich

Octyl β-D-glucopyranoside solution

≥95% (HPLC), 50 % (w/v) in H2O

Synonym(s):

n-Octyl glucoside, OGP

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About This Item

Empirical Formula (Hill Notation):
C14H28O6
CAS Number:
Molecular Weight:
292.37
MDL number:
UNSPSC Code:
12161902
PubChem Substance ID:
NACRES:
NB.22

description

non-ionic

Assay

≥95% (HPLC)

mol wt

average mol wt 24500-25000

concentration

50 % (w/v) in H2O

aggregation number

84

CMC

20-25

transition temp

cloud point ≥100

application(s)

detection

storage temp.

−20°C

SMILES string

CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O

InChI

1S/C14H28O6/c1-2-3-4-5-6-7-8-19-14-13(18)12(17)11(16)10(9-15)20-14/h10-18H,2-9H2,1H3/t10-,11-,12+,13-,14-/m1/s1

InChI key

HEGSGKPQLMEBJL-RKQHYHRCSA-N

Application

2.5% n-octyl β-D glucopyranoside has been used to homogenize mouse retina
n-Octyl β-D-glucopyranoside (OGP) is a non-ionic detergent which has been used for isoelectric focusing (IEF) and two-dimensional electrophoresis (2D). OGP has been shown to be superior to Triton X-100 for IEF of plant proteins. n-Octyl β-D-glucopyranoside has also been used for concentration of protein from 2D gels for digestion and mass spectroscopy analysis.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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P Dainese Hatt et al.
European journal of biochemistry, 246(2), 336-343 (1997-06-01)
We have developed a gel electrophoresis system that can concentrate proteins from spots cut out of up to 50 two-dimensional electrophoresis gels. During protein concentration, SDS is substituted with a non-ionic detergent (octyl beta-glucopyranoside) which allows digestion and MS analysis
P J Holloway et al.
Analytical biochemistry, 172(1), 8-15 (1988-07-01)
A technique for the analysis of plant proteins from seed, leaf, root, and coleoptile tissues by high resolution two-dimensional electrophoresis is described. This technique is based primarily on the procedure of P. O'Farrell (1975, J. Biol. Chem. 250, 4007-4021); however
M F Lopez
Journal of chromatography. B, Biomedical sciences and applications, 722(1-2), 191-202 (1999-03-06)
Two-dimensional electrophoresis has rapidly become the method of choice for resolving complex mixtures of proteins. Since the technique was pioneered in 1975, 2-D gel methods have undergone a series of enhancements to optimize resolution and reproducibility. Recent improvements in the
Compartment-specific phosphorylation of phosducin in rods underlies adaptation to various levels of illumination.
Song H, et al.
The Journal of Biological Chemistry (2007)
Zahrabatoul Mosapour Kotena et al.
Journal of molecular modeling, 19(2), 589-599 (2012-09-14)
Density functional theory calculations on two glycosides, namely, n-octyl-β-D-glucopyranoside (C(8)O-β-Glc) and n-octyl-β-D-galactopyranoside (C(8)O-β-Gal) were performed for geometry optimization at the B3LYP/6-31G level. Both molecules are stereoisomers (epimers) differing only in the orientation of the hydroxyl group at the C4 position.

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