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Key Documents

P3X63Ag8

85011401, mouse skin, Lymphoblast

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About This Item

UNSPSC Code:
41106514

product name

P3X63Ag8, 85011401

biological source

mouse skin

growth mode

Suspension

karyotype

Aneuploid

morphology

Lymphoblast

products

Immunoglobulin G (IgG1) (kappa)

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse myeloma

Cell Line Description

Derived from the P3X27 cell line, itself a derivative of mineral oil-induced plasmacytoma MOPC-21 in BALB/c mice. Resistant to 20μg/ml azaguanine, does not grow in selective medium. HGPRT neg., dies in HAT. Doubling time 16-26 h.

Application

Fusion partner

Culture Medium

DMEM or RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 5x10,000 to 3x100,000 cells/ml; 5% CO2; 37°C. Cells will achieve maximum cell density of 5x100,000 cells/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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Articles

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

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