KCQS06

KiCqStart^® Probe qPCR ReadyMix^™, ROX^™

with ROX for ABI instruments

• Synonym(s): Kicqstart^® Probe qPCR ReadyMix^™
• UNSPSC Code: 41106300
• NACRES: NA.55

Properties

• form: liquid
• usage: sufficient for 1250 reactions; sufficient for 250 reactions; sufficient for 5000 reactions
• feature: dNTPs included; hotstart
• storage condition: protect from light
• technique(s): qPCR: suitable
• color: light blue
• input: purified DNA
• compatibility: ABI 5700; ABI 7000; ABI 7300; ABI 7700; ABI 7900 HT Fast; ABI 7900 HT; ABI 7900; ABI StepOne; ABI StepOnePlus
• detection method: probe-based
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

KiCqStart^® Probe qPCR ReadyMix^™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan^® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. This PCR master mix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Application

KiCqStart^® Probe qPCR ReadyMix^™, ROX^™ has been used for amplification of cDNA of a stemness markers genes by quantitative reverse transcription-polymerase chain reaction (RT-qPCR).

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye.

Features and Benefits

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers

Legal Information

KiCqStart is a registered trademark of QIAGEN Beverly Inc.

ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable