MiCL1 (S+L-)

86020603, mink lung, Fibroblast-like

• UNSPSC Code: 41106514

Properties

• product name: MiCL1 (S+L-), 86020603
• biological source: mink lung
• growth mode: Adherent
• karyotype: 2n = 30
• morphology: Fibroblast-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mink lung, Moloney murine sarcoma virus infected

Cell Line Description

A clone of the mink lung cell line Mv.1.Lu (Sigma Catalogue number. 88050503). Isolated from a single cell of a focus produced in the monolayer after infection with Moloney murine sarcoma virus associated with the RD-114 helper leukaemia virus. Super infection with non-transforming leukaemia virus results in transformation and net amplification of sarcoma-specific RNA sequences.

Application

Virus studies: Moloney MSV and RD-114 helper leukaemia virus Leukemogenesis

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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