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product name
EL4.BU.OU6, 85121301
biological source
mouse blood
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Interleukin 2 (IL-2), Interleukin 4 (IL-4), Thy-1.2
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse C57BL/6N ascites lymphoma lymphoblast
Cell Line Description
Derivative of EL4.BU. Resistant to BUdR and ouabain and expresses Thy-1.2 Ag. Will die in HAT selective medium. A high producer of interleukin 2 and interleukin 4.
Application
Resistance and hormone studies
Culture Medium
DMEM + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 1.16g/l 1% L-Arginine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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