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Key Documents

393A-1

Sigma-Aldrich

Adipophilin Rabbit Polyclonal Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100
500

conjugate

unconjugated

antibody form

Ig fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (393A-14)
vial of 0.5 mL concentrate (393A-15)
bottle of 1.0 mL predilute (393A-17)
vial of 1.0 mL concentrate (393A-16)
bottle of 7.0 mL predilute (393A-18)

manufacturer/tradename

Cell Marque

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100

control

sebaceous neoplasms (intracytoplasmic lipid droplet)

shipped in

wet ice

storage temp.

2-8°C

visualization

membranous

Gene Information

human ... PLIN2(123)

General description

Sebaceous carcinoma is a relatively uncommon cutaneous malignancy and mimics other malignant neoplasms, such as basal and squamous cell carcinomas, and benign processes, such as chalazions and blepharitis, sometimes resulting in delayed diagnosis and suboptimal treatment.Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various normal cell types. Recently, it has been reported that adipophilin was expressed in 16 of 16 (100%) sebaceous adenomas with a specific pattern: membranous with strong uptake at the periphery of intracytoplasmic lipid vacuoles.

Of 25 sebaceous carcinomas, 23 (92%) were also labeled with a similar pattern. Additionally, in cases of poorly differentiated sebaceous carcinoma (n=11), in which sebaceous differentiation could not have been reliably interpreted in H&E sections, adipophilin highlighted the sebocytes with a strong membranous labeling of intracytoplasmic lipid droplets in 9 of 11 cases (82%). Moreover, 10 of 10 (100%) xanthelasmas, 9 of 10 (90%) xanthogranulomas, 6 of 6 (100%) xanthomas, and 9 of 13 (63%) metastatic renal cell carcinomas were also weakly-to- moderately positive for adipophilin. Expression of adipophilin with a membranous pattern of staining was not seen in any of the other clear cell lesions of the skin, including basal and squamous cell carcinomas, trichilemmomas, clear cell hidradenomas, or balloon cell nevi. Interestingly, a nonspecific granular uptake of anti-adipophilin was seen in adjacent macrophages, keratohyalin granules of epithelial squamous cells, and some tumor cells. Therefore, this anti-adipophilin is suitable for immunostaining formalin-fixed, paraffin-embedded tissue and is helpful in the identification of intracytoplasmic lipids, as seen in sebaceous lesions. It is especially helpful in identifying intracytoplasmic lipid vesicles in poorly differentiated sebaceous carcinomas in challenging cases such as small periocular biopsy specimens.

Quality


IVD

IVD

IVD

RUO

Linkage

Adipophilin Positive Control Slides, Product No. 393S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

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Mohamed Ali Alabiad et al.
Iranian journal of pathology, 16(2), 162-173 (2021-05-04)
Diagnosis and discrimination of lung adenocarcinoma (LUAD) from lung squamous cell carcinoma (LUSC) is critical to select the appropriate treatment regimen as recently targeted therapies require accurate subtyping of nonsmall-cell lung carcinoma (NSCLCs). There are currently several biomarkers that could
Sarah A Donnell et al.
Journal of cutaneous pathology, 48(10), 1252-1261 (2021-05-06)
We and others have noticed consistent staining of sebaceous glands with PReferentially expressed Antigen in MElanoma (PRAME). We aimed to determine whether PRAME was as sensitive, specific, and interpretable as adipophilin for distinguishing sebaceous neoplasms (SNs) from other neoplasms. Twenty
Tze F Lai et al.
Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], 30(2 Pt 1), 222-225 (2004-02-06)
Sebaceous carcinoma (SC) accounts for 1% to 5.5% of all eyelid malignancies. Diagnosis is often delayed because of its ability to masquerade as other periocular lesions both clinically and histologically. To promote a high index of suspicion among Mohs surgeons
Daniel A Ostler et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 23(4), 567-573 (2010-02-02)
Adipophilin is a monoclonal antibody against a protein on the surface of intracellular lipid droplets, and it was recently shown to be expressed in sebocytes and sebaceous lesions. This study examines adipophilin expression in various sebaceous lesions and other cutaneous

Articles

IHC antibodies enhance dermatopathology beyond H&E stained slides, improving techniques and applications for dermatological research.

IHC antibodies enhance dermatopathology beyond H&E stained slides, improving techniques and applications for dermatological research.

IHC antibodies enhance dermatopathology beyond H&E stained slides, improving techniques and applications for dermatological research.

IHC antibodies enhance dermatopathology beyond H&E stained slides, improving techniques and applications for dermatological research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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