Recombinant, Arthrobacter ureafaciens α2-3,6,8,9-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. High concentrations of this sialidase and prolonged periods of incubation result in the additional cleavage of branched sialic acids. This enzyme is found as two active species of MW 60000 and 69000.
Warning
Toxicity: Harmful (C)
Unit Definition
One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid per min at 37°C, pH 5.0. (Note: 1 mU = 1 milliunit).
Physical form
In 20 mM Tris-HCl, 20 mM NaCl, pH 7.5.
Other Notes
Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181. Prime, S., et al. 1996. J. Chromatogr. A. 720, 263. Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569 Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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