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  • Phase-Contrast Radiography Enables Detection of Early Changes in Articular Cartilage in a Mouse Model of Osteoarthritis.

Phase-Contrast Radiography Enables Detection of Early Changes in Articular Cartilage in a Mouse Model of Osteoarthritis.

American journal of physical medicine & rehabilitation (2014-11-22)
Joon Shik Yoon, Sun Jae Won, Sei Joo Kim, Suk Joo Hong, Gui Sang Kim, Jae Min Kim
ABSTRACT

The aim of this study was to investigate the potential of using phase-contrast radiography to detect early changes in articular cartilage in a mouse model of osteoarthritis. An iodoacetate-induced mouse model of osteoarthritis was used. Mice with iodoacetate-induced osteoarthritis were euthanized at 1 and 2 wks after injection. Specimens were obtained by sectioning mouse tissue into 5-mm-thick slices. Sections were examined using synchrotron x-rays from the 7B2 beamline at the Pohang Light Source. Magnified microradiographic images, obtained by phase-contrast radiography, were used to identify changes in articular cartilage and the subchondral bone. Refraction-enhanced phase-contrast radiography enabled detailed microscopic and macroscopic observation of articular cartilage by a noninvasive technique. Detailed microscopic images revealed partially damaged articular cartilage, containing an irregular trilaminar zone, at 1 wk after injection. At 2 wks after injection, a necrotic cleft was clearly visible, along with collapse of the trilaminar zones. Shrunken chondrocytes and chondrocyte clusters of variable sizes were also observed. Phase-contrast radiography is a feasible technique for the early detection of osteoarthritis and is capable of detecting microstructural changes in articular cartilage.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium iodoacetate, ≥98%
Sigma-Aldrich
Sodium iodoacetate, ≥98%, BioUltra
Sigma-Aldrich
Sodium iodoacetate, BioUltra, ≥99.5% (NT)