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  • Scleral permeability varies by mouse strain and is decreased by chronic experimental glaucoma.

Scleral permeability varies by mouse strain and is decreased by chronic experimental glaucoma.

Investigative ophthalmology & visual science (2014-02-22)
Mary E Pease, Ericka N Oglesby, Elizabeth Cone-Kimball, Joan L Jefferys, Matthew R Steinhart, Anthony J Kim, Justin Hanes, Harry A Quigley
ABSTRACT

To determine differences in scleral permeability, as measured by diffusion of macromolecules, by using fluorescence recovery after photobleaching (FRAP), with reference to differences by mouse strain, scleral region, and the effect of experimental glaucoma. In three mouse strains (B6, CD1, and B6 mice with mutation in collagen 8α2 [Aca23]), we used FRAP to measure the diffusion of fluorescein isothiocyanate-dextran, molecular weight 40 kDa, into a photobleached zone of sclera. Scleral regions near the optic nerve head (peripapillary) and two successively more anterior regions were compared. Sclera from mouse eyes subjected to chronically elevated intraocular pressure after bead injection into the anterior chamber were compared to fellow eye controls. FRAP data were compared against estimated retinal ganglion cell axon loss in glaucomatous eyes. Diffusion rates of dextran molecules in the sclera were significantly greater in Aca23 and B6 mice than in CD1 mice in a multivariate model adjusted for region and axial length (P < 0.0001). Dextran diffusion significantly decreased in glaucomatous eyes, and the decline increased with greater axon loss (P = 0.0003, multivariable model). Peripapillary scleral permeability was higher in CD1 than B6 and Aca23 mice (P < 0.05, multivariable model, adjusted by Bonferroni). Measurement of the diffusion rates of dextran molecules in the sclera showed that glaucoma leads to decreased scleral permeability in all three mouse strains tested. Among mouse strains tested, those that were more susceptible to glaucomatous loss of retinal ganglion cells had a lower scleral permeability at baseline.

MATERIALS
Product Number
Brand
Product Description

Dextran 1, Dextran 1 European Pharmacopoeia (EP) Reference Standard
Dextran Vo, Dextran Vo European Pharmacopoeia (EP) Reference Standard
Dextran 4 for calibration, Dextran 4 European Pharmacopoeia (EP) Reference Standard
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Dextran from Leuconostoc spp., Mr ~100,000
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Dextran from Leuconostoc mesenteroides, average mol wt 60,000-76,000
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Dextran from Leuconostoc mesenteroides, average mol wt 15,000-30,000
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Dextran from Leuconostoc mesenteroides, average mol wt 48,000-90,000
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Dextran from Leuconostoc mesenteroides, average mol wt 9,000-11,000
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Dextran from Leuconostoc mesenteroides, average mol wt 35,000-45,000
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Dextran solution from Leuconostoc mesenteroides, 20 % (w/w) (Autoclaved)
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Dextran from Leuconostoc spp., Mr ~40,000
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Dextran from Leuconostoc mesenteroides, average mol wt 1,500,000-2,800,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 410,000
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Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 670,000
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Dextran from Leuconostoc mesenteroides, for GPC, analytical standard, Mw 50,000
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Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 12,000
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Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 5,000
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Dextran from Leuconostoc spp., Mr ~70,000
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Dextran, enzymatic synth.
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Dextran, analytical standard, for GPC, 410,000
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Dextran, analytical standard, for GPC, 25,000
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Dextran, analytical standard, for GPC, 670,000
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Dextran, analytical standard, for GPC, 270,000
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Dextran, analytical standard, for GPC, 1,000
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Dextran from Leuconostoc spp., Mr 450,000-650,000
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Dextran from Leuconostoc spp., Mr ~6,000
Supelco
Dextran, analytical standard, for GPC, 12,000
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Dextran from Leuconostoc spp., Mr 15,000-25,000
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Dextran, analytical standard, for GPC, 5,000
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Dextran, analytical standard, for GPC, 150,000