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  • Dextran-functionalized magnetic fluid mediating magnetohyperthermia for treatment of Ehrlich-solid-tumor-bearing mice: toxicological and histopathological evaluations.

Dextran-functionalized magnetic fluid mediating magnetohyperthermia for treatment of Ehrlich-solid-tumor-bearing mice: toxicological and histopathological evaluations.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (2013-12-03)
Ana Luisa Miranda-Vilela, Kelly Reis Yamamoto, Kely Lopes Caiado Miranda, Breno Noronha Matos, Marcos Célio de Almeida, João Paulo Figueiró Longo, José de Souza Filho, Juliana Menezes Soares Fernandes, Patrícia Pommé Confessori Sartoratto, Zulmira Guerrero Marques Lacava
ABSTRACT

Dextran-functionalized maghemite fluid (DexMF) has been tested to treat Ehrlich-solid-tumor-bearing mice, evidencing its potential use in mediating magnetohyperthermia in breast cancer treatment. However, although magnetic nanoparticles tend to accumulate in tumor tissues, part of the nanomaterial can reach the blood stream, and then the organism. The aim of this study was to investigate the acute systemic effects of the intratumoral injection of DexMF mediating magnetohyperthermia in the treatment of an advanced clinical Ehrlich-solid-tumor, assessed through histopathological analyses of liver, kidneys, heart and spleen, comet assay, micronucleus test, hemogram, and serum levels of bilirubin, aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transferase, alkaline phosphatase, creatinine, and urea. The tumor's histopathology and morphometry were used to assess its aggressiveness and regression. DexMF mediating hyperthermia was effective in containing tumor aggressiveness and in inducing tumor regression, besides showing no toxic effects. Its physical characteristics also suggest that it is safe to use in other biomedical applications.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~100,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 60,000-76,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 15,000-30,000
Sigma-Aldrich
γ-Glutamyltranspeptidase from porcine kidney, Type IV, 2.0-6.0 units/mg solid
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 48,000-90,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 9,000-11,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 35,000-45,000
Sigma-Aldrich
Dextran solution from Leuconostoc mesenteroides, 20 % (w/w) (Autoclaved)
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~40,000
Sigma-Aldrich
γ-Glutamyltranspeptidase from equine kidney, Type VI, 5-12 units/mg solid
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, average mol wt 1,500,000-2,800,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 410,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 670,000
Supelco
Dextran from Leuconostoc mesenteroides, for GPC, analytical standard, Mw 50,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 5,000
Supelco
Dextran from Leuconostoc mesenteroides, analytical standard, for GPC, Mw 12,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~70,000
Sigma-Aldrich
Dextran, enzymatic synth.
Supelco
Dextran, analytical standard, for GPC, 410,000
Supelco
Dextran, analytical standard, for GPC, 25,000
Supelco
Dextran, analytical standard, for GPC, 670,000
Supelco
Dextran, analytical standard, for GPC, 270,000
Supelco
Dextran, analytical standard, for GPC, 1,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr 450,000-650,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr ~6,000
γ-Glutamyl Transferase, ERM®, certified reference material
Supelco
Dextran, analytical standard, for GPC, 5,000
Sigma-Aldrich
Dextran from Leuconostoc mesenteroides, Mr ~200,000
Supelco
Dextran, analytical standard, for GPC, 12,000
Sigma-Aldrich
Dextran from Leuconostoc spp., Mr 15,000-25,000