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Key Documents

SRP0300

Sigma-Aldrich

TGF-β1 Latent human

recombinant, expressed in FreeStyle™ 293-F cells, ≥98% (SDS-PAGE)

Synonym(s):

LAP, TGFb1, TGFbeta1, latency-associated peptide, transforming growth factor beta-1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in FreeStyle™ 293-F cells

Assay

≥98% (SDS-PAGE)

form

aqueous solution

mol wt

25 kDa

packaging

pkg of 5 μg

manufacturer/tradename

Invitrogen

storage condition

avoid repeated freeze/thaw cycles

NCBI accession no.

NM_000660

UniProt accession no.

Q14766

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... LTBP1(4052)

General description

Human TGFβ1 (GenBank Accession No. NM_000660), amino acids 30-390 (end) with Cys33Ser mutation and N-terminal His-tag, disulfide-linked homodimers of TGFβ1 LAP and mature TGFβ1, MW= ~75kDa and 25kDa, under non-reducing conditions.
LTBP1 (latent transforming growth factor β binding protein 1) belongs to the LTBP family of proteins. The protein has 18 epidermal growth factor (EGF)-like repeats and four LTBP-like repeats. The gene is mapped to human chromosome 2p22.3.

Application

Useful for cell culture and for the study of signaling and apoptotic pathways.

Biochem/physiol Actions

LTBP1 (latent transforming growth factor β binding protein 1) is an extracellular matrix protein which also controls TGF-β (transforming growth factor β) bioavailability. LTBP1 is mainly responsible for the folding and secretion of TGF-β, thereby adding it to the extracellular matrix. It is a target of HTRA1 (high temperature requirement protein A1), a serine protease involved in cellular signaling.

Physical form

Lyophilized from a 0.2 μM filtered, aqueous solution.

Reconstitution

Reconstitute in sterile PBS with 0.1% BSA. Stability: >6 months at –70°C (as a solid). Store reconstituted protein in aliquots at –80°C for up to 3 months. Avoid freeze/thaw cycles.

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Association of the small latent transforming growth factor-beta with an eight cysteine repeat of its binding protein LTBP-1.
Saharinen J, et al.
The Embo Journal, 15, 245-253 (1996)
A suite of novel human spermatozoal RNAs.
Ostermeier GC, et al.
Journal of Andrology, 26, 70-74 (2005)
Cerebral small vessel disease-related protease HtrA1 processes latent TGF-? binding protein 1 and facilitates TGF-? signaling.
Beaufort N, et al.
Proceedings of the National Academy of Sciences of the USA, 111, 16496-16501 (2014)
R Derynck et al.
Nature, 316(6030), 701-705 (1985-08-22)
The partial amino-acid sequence of purified human transforming growth factor-beta (TGF-beta) was used to identify a series of cDNA clones encoding the protein. The cDNA sequence indicates that the 112-amino acid monomeric form of the natural TGF-beta homodimer is derived
L Bourdrel et al.
Protein expression and purification, 4(2), 130-140 (1993-04-01)
A partial cDNA clone encoding the gene for human transforming growth factor-beta 1 (TGF-beta 1) was isolated from a human bladder carcinoma cell line (5637) cDNA library. Following restriction enzyme processing and ligation of synthetic oligonucleotide linkers, the gene was
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