487929-M

Nocodazole

InSolution, ≥97%, 50 mM in DMSO, Inhibitor of mammalian cell mitosis via a highly aspecific antimicrotubular activity

• Synonym(s): InSolution Nocodazole, Methyl-(5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl)-carbamate, Methyl-5-(thiophene-2-carbonyl)-1H-benzo[d]imidazol-2-ylcarbamate, R17934, R17934, Methyl-(5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl)-carbamate, Methyl-5-(thiophene-2-carbonyl)-1H-benzo[d]imidazol-2-ylcarbamate
• Empirical Formula (Hill Notation): C₁₄H₁₁N₃O₃S
• CAS Number: 31430-18-9
• Molecular Weight: 301.32
• MDL number: MFCD00005588
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥97% (HPLC)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• storage temp.: −70°C
• InChI: 1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19)
• InChI key: KYRVNWMVYQXFEU-UHFFFAOYSA-N

Description

General description

Inhibitor of mitosis. Has highly specific antimicrotubular activity for mammalian cells in culture. Promotes tubulin depolymerization. Also acts as a high-affinity ligand for the cancer-related kinases ABL, c-KIT, BRAF, and MEK (K_d<2 M). The solid form of this compound (Cat. No. 487928) is also available.

Biochem/physiol Actions

Primary Target
Microtubules

Secondary Target
Certain kinases

Warning

Toxicity: Irritant (B)

Physical form

A 50 mM (10 mg/664 µL) solution of Nocodazole (Cat. No. 487928) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.

Other Notes

Park, H., et al. 2012. Chem. Med. Chem.7, 53.
Bunz, F., et al. 1998. Science282, 1497.
Fleischman, L.F., et al. 1993. Oncogene8, 771.Pomies, P., and Block, M.R. 1992. Biochem. Biophys. Res. Commun.189, 1429.
Rennison, M.E., et al. 1992. J. Cell Sci.102, 239.
Thyberg, J., and Moskalewski, S. 1992. J. Cell Sci.103, 1167.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H341
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): 188.6 °F - closed cup - (refers to pure substance)
• Flash Point(C): 87 °C - closed cup - (refers to pure substance)