05-689
Anti-HP1α Antibody, clone15.19s2
clone 15.19s2, Upstate®, from mouse
Synonym(s):
Antigen p25, HP1 alpha, HP1Hs alpha, Heterochromatin protein 1 homolog alpha, chromobox homolog 5 (Drosophila HP1 alpha), chromobox homolog 5 (HP1 alpha homolog, Drosophila), heterochromatin protein 1-alpha
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
15.19s2, monoclonal
species reactivity
mouse, vertebrates, human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CBX5(23468)
Related Categories
General description
Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and each contains a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated on Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence-independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases and the p150 subunit of chromatin-assembly factor-1 (CAF1). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells. HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination and sumoylation, suggesting multiple means of regulation.
Specificity
This antibody recognizes HP1α
Immunogen
GST fusion protein corresponding to residues 2-191 of human HP1α protein.
Application
Anti-HP1α Antibody, clone15.19s2 is a Mouse Monoclonal Antibody for detection of HP1α also known as HP1Hs alpha, Heterochromatin protein 1 homolog alpha & has been validated in ChIP, ICC, IHC, IP & WB.
Immunohistochemistry:
A previous lot of this antibody has been reported by an independent laboratory to detect HP1a in NIH/3T3 nuclei (Ayyanathan, K., 2003).
Immunoprecipitation/Chromatin Immunoprecipitation:
A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate HP1α from nuclear extracts and formalin-cross-linked chromatin (Ayyanathan, K., 2003).
A previous lot of this antibody has been reported by an independent laboratory to detect HP1a in NIH/3T3 nuclei (Ayyanathan, K., 2003).
Immunoprecipitation/Chromatin Immunoprecipitation:
A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate HP1α from nuclear extracts and formalin-cross-linked chromatin (Ayyanathan, K., 2003).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
RNA Binding Protein (RBP)
Chromatin Biology
RNA Binding Protein (RBP)
Quality
Routinely evaluated by western blot in acid-extracted HeLa cell lysates.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected HP1α protein in acid-extracted HeLa cell lysates.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected HP1α protein in acid-extracted HeLa cell lysates.
Target description
29 kDa
Linkage
Replaces: MABE479
Physical form
Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa, NIH/3T3 and COS cell lysates.
HeLa, NIH/3T3 and COS cell lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Due to license agreement restrictions, this product cannot be purchased for resale.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Loss of heterochromatin protein 1 gamma reduces the number of primordial germ cells via impaired cell cycle progression in mice.
Abe, K; Naruse, C; Kato, T; Nishiuchi, T; Saitou, M; Asano, M
Biology of reproduction null
Histone modification in constitutive heterochromatin versus unexpressed euchromatin in human cells.
Guanchao Jiang, Fan Yang, Cecilia Sanchez, Melanie Ehrlich
Journal of Cellular Biochemistry null
Eva Bártová et al.
Differentiation; research in biological diversity, 76(1), 24-32 (2007-06-19)
Human embryonic stem cells (hES) are unique in their pluripotency and capacity for self-renewal. Therefore, we have studied the differences in the level of chromatin condensation in pluripotent and all-trans retinoic acid-differentiated hES cells. Nuclear patterns of the Oct4 (6p21.33)
Differentiation-independent fluctuation of pluripotency-related transcription factors and other epigenetic markers in embryonic stem cell colonies.
Sustackova, G; Legartova, S; Kozubek, S; Stixova, L; Pachernik, J; Bartova, E
Stem Cells and Development null
Anne-Sophie Huart et al.
PloS one, 7(8), e43391-e43391 (2012-08-24)
Protein-protein interactions forming dominant signalling events are providing ever-growing platforms for the development of novel Biologic tools for controlling cell growth. Casein Kinase 1 α (CK1α) forms a genetic and physical interaction with the murine double minute chromosome 2 (MDM2)
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