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Sigma-Aldrich

Anti-phospho-Insulin Receptor (Tyr 1322) Antibody, clone 21G12

clone 21g12, Upstate®, from mouse

Synonym(s):

INSR

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

21g12, monoclonal

species reactivity

canine, human, mouse

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pTyr1322)

Gene Information

human ... INSR(3643)

Specificity

Recognizes phosphorylated domain of Insulin receptor on Tyrosine 1322.

Immunogen

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyrosine 1322 on phosphorylated insulin receptor

Application

Anti-phospho-Insulin Receptor Antibody, clone 21G12 is an antibody against phospho-Insulin Receptor (Tyr 1322) for use in ELISA & WB.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Quality

Routinely evaluated by immunoblot.

Target description

97 kDa

Physical form

Format: Purified
Lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.
Subsequent thiophilic adsorption and size exclusion chromatography

Storage and Stability

2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Includes pervanadate treated HEK293 lysate as a positive control.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Francesca Fiory et al.
Molecular and cellular biology, 25(24), 10803-10814 (2005-11-30)
In L6 myoblasts, insulin receptors with deletion of the C-terminal 43 amino acids (IR(Delta43)) exhibited normal autophosphorylation and IRS-1/2 tyrosine phosphorylation. The L6 cells expressing IR(Delta43) (L6(IRDelta43)) also showed no insulin effect on glucose uptake and glycogen synthase, accompanied by
Molecular cloning of a DNA sequence complementary to creatine kinase M mRNA from chickens.
Schweinfest, CW; Kwiatkowski, RW; Dottin, RP
Proceedings of the National Academy of Sciences of the USA null
Frankie B Stentz et al.
Biochemical and biophysical research communications, 335(2), 491-495 (2005-08-09)
Upon activation by phytohemagglutine (PHA), T-lymphocytes (T-cells) express receptors for growth factors, insulin, IGF-1 and IL2 and become insulin sensitive. Diabetic ketoacidosis (DKA) is associated with in vivo emergence of these growth factor receptors without incubation with PHA. As DKA
Qiu-yan Wang et al.
The FEBS journal, 274(2), 526-538 (2007-01-19)
Two H7721 human hepatocarcinoma cell lines showing moderate and high expression of alpha1,3-fucosyltransferase (FucT)-VII cDNA were established and designated FucTVII-M and FucTVII-H, respectively. In alpha1,3-FucT-VII-transfected cells, expression of insulin receptor (InR) alpha- and beta subunits and epidermal growth factor receptor
Template to improve glycemic control without reducing adiposity or dietary fat.
Krishnapuram, R; Dhurandhar, EJ; Dubuisson, O; Kirk-Ballard, H; Bajpeyi, S; Butte et al.
American Journal of Physiology. Endocrinology and Metabolism null

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