Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

215430

Sigma-Aldrich

α-(4-Pyridyl N-oxide)-N-tert-butylnitrone

99%

Synonym(s):

N-(4-Pyridylmethylene)-tert-butylamine N,N′-dioxide, N-tert-Butyl-α-(4-pyridyl)nitrone N′-oxide, POBN

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

Empirical Formula (Hill Notation):
C10H14N2O2
CAS Number:
66893-81-0
Molecular Weight:
194.23
Beilstein:
1453764
EC Number:
266-512-3
MDL number:
MFCD00041022
UNSPSC Code:
12352100
PubChem Substance ID:
24852894
NACRES:
NA.22

Assay

99%

form

solid

mp

183-185 °C (lit.)

solubility

water: soluble 10 mg/mL, clear, colorless to faintly yellow

SMILES string

CC(C)(C)\[N+]([O-])=C/c1cc[n+]([O-])cc1

InChI

1S/C10H14N2O2/c1-10(2,3)12(14)8-9-4-6-11(13)7-5-9/h4-8H,1-3H3/b12-8+

InChI key

RNRMWTCECDHNQU-XYOKQWHBSA-N

Looking for similar products? Visit Product Comparison Guide

Application

α-(4-Pyridyl N-oxide)-N-tert-butylnitrone was used as spin trapping agent to detect:
  • the formation of hydroxyl radical adducts during decomposition of hydrogen peroxide in the presence of nickel (II) oligopeptides
  • hydroxyl radicals and selenide radical anions
Spin trap reagent used to measure ethanol oxidation to 1-hydroxyethyl radical (HER) which can bind to protein. Has also been used in studies of DNA methylation, iron-mediated polyunsaturated fatty acid peroxidation, and free-radical formation by Alzheimer β-amyloid peptide. The formation of hydroxyl radical in chondrocytes and cartilage has been detected using POBN.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Bulletin of the Chemical Society of Japan, 64, 1976-1976 (1991)
Measurement of hydroxyl radical activity in a soil slurry using the spin trap a-(4-pyridyl-1-oxide)-N-tert-butylnitrone.
Huling SG, et al.
Environmental Science & Technology, 32(21), 3436-3441 (1998)
J. Photochem. Photobiol., A, 56, 89-89 (1991)
Andrew E Maclean et al.
PLoS pathogens, 17(3), e1009301-e1009301 (2021-03-03)
The mitochondrial electron transport chain (mETC) and F1Fo-ATP synthase are of central importance for energy and metabolism in eukaryotic cells. The Apicomplexa, important pathogens of humans causing diseases such as toxoplasmosis and malaria, depend on their mETC in every known
S Inoue et al.
Biochemical and biophysical research communications, 159(2), 445-451 (1989-03-15)
ESR studies using spin traps, 5,5-dimethylpyrroline-N-oxide and alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone, revealed that hydroxyl radical adducts are produced by the decomposition of hydrogen peroxide in the presence of nickel(II) oligopeptides. Order of catalytic activities of nickel(II) oligopeptides used in the production of
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service