Amicon^® Pro Purification System – Protein Purification

The Amicon^® Pro Purification System is an all-in-one centrifugal device for the protein purification workflow. Traditional protein purification is a long process with many steps and multiple tubes, often resulting in protein degradation and loss. Avoid the risks associated with sample transfer and reduce hands-on time when you bind, wash, elute and/or concentrate your protein in the all-in-one Amicon^® Pro purification system. Regardless of your workflow, this virtually touchless device delivers consistent, accurate sample preparation, resulting in reliable recovery, uncompromised purity and easy data generation.

The Amicon^® Pro Purification System Workflow

Bind, wash, elute and/or concentrate your protein in this all-in-one centrifugal device.

Elute, Don't Dilute

Excellent Recovery, Regardless of Scale

Figure 1. While three elution steps were required for the smaller spin columns (left), a single spin in the Amicon^® Pro device was sufficient to recover > 90% of resin-bound protein in an average of 45 µL final volume*.

Figure 2. Protein recovered increases linearly with respect to packed resin volume, offering flexibility in experimental scale. Whether you are using a few microliters or a milliliter of resin, Amicon^® Pro purification yields consistent recovery.

Compare the Amicon^® Pro Workflow with Traditional Methods

Purification with buffer exchange/concentration			Purification only
0.5 mL spin column + concentration device		Amicon® Pro Purification System	0.5 mL gravity column	Amicon® Pro Purification System
Process time	67 minutes	29 minutesa	125 minutesb	65 minutesa
Steps required	12	6	18	6
Sample transfers	1	0	3-8 (# of fractions collected)	0

^a Process time excludes 60 minute incubation for both protocols.
^b Gravity column process includes column setup, purification and assessment of eluted fractions.