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  • Facile One-step Micropatterning Using Photodegradable Methacrylated Gelatin Hydrogels for Improved Cardiomyocyte Organization and Alignment.

Facile One-step Micropatterning Using Photodegradable Methacrylated Gelatin Hydrogels for Improved Cardiomyocyte Organization and Alignment.

Advanced functional materials (2015-09-04)
Kelly M C Tsang, Nasim Annabi, Francesca Ercole, Kun Zhou, Daniel Karst, Fanyi Li, John M Haynes, Richard A Evans, Helmut Thissen, Ali Khademhosseini, John S Forsythe
ABSTRACT

Hydrogels are often employed as temporary platforms for cell proliferation and tissue organization in vitro. Researchers have incorporated photodegradable moieties into synthetic polymeric hydrogels as a means of achieving spatiotemporal control over material properties. In this study protein-based photodegradable hydrogels composed of methacrylated gelatin (GelMA) and a crosslinker containing o-nitrobenzyl ester groups have been developed. The hydrogels are able to degrade rapidly and specifically in response to UV light and can be photopatterned to a variety of shapes and dimensions in a one-step process. Micropatterned photodegradable hydrogels are shown to improve cell distribution, alignment and beating regularity of cultured neonatal rat cardiomyocytes. Overall this work introduces a new class of photodegradable hydrogel based on natural and biofunctional polymers as cell culture substrates for improving cellular organization and function.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gelatin methacryloyl, gel strength 300 g Bloom, degree of substitution 40%
Sigma-Aldrich
Gelatin methacryloyl, gel strength 300 g Bloom, degree of substitution 60%
Sigma-Aldrich
Gelatin methacryloyl, gel strength 90-110 g Bloom, degree of substitution 60%
Sigma-Aldrich
Low endotoxin GelMA, bloom 300, Type A, degree of substitution 80%
Sigma-Aldrich
Low endotoxin GelMA, gel strength 300 (Bloom), degree of substitution 80%, GelMA Type B
Sigma-Aldrich
Low endotoxin GelMA, mol wt 95 kDa, degree of substitution 60%
Sigma-Aldrich
Gelatin acrylate, gel strength 300 g Bloom, degree of substitution 60%
Sigma-Aldrich
Low endotoxin GelMA solution, gel strength 300 (bloom), degree of substitution 80%, 0.2 μm, sterile-filtered, GelMA Type B