- In vitro activities of amoxicillin-clavulanate, doxycycline, ceftazidime, imipenem, and trimethoprim-sulfamethoxazole against biofilm of Brazilian strains of Burkholderia pseudomallei.
In vitro activities of amoxicillin-clavulanate, doxycycline, ceftazidime, imipenem, and trimethoprim-sulfamethoxazole against biofilm of Brazilian strains of Burkholderia pseudomallei.
Antimicrobial agents and chemotherapy (2013-09-05)
Tereza de Jesus Pinheiro Gomes Bandeira, Camila Alencar Moreira, Raimunda Sâmia Nogueira Brilhante, Débora de Souza Collares Maia Castelo-Branco, Manoel Paiva de Araújo Neto, Rossana de Aguiar Cordeiro, Terezinha de Jesus Santos Rodrigues, Marcos Fábio Gadelha Rocha, José Júlio Costa Sidrim
PMID24002089
ABSTRACT
This study aimed at investigating the in vitro activities of amoxicillin-clavulanate, doxycycline, ceftazidime, imipenem, and trimethoprim-sulfamethoxazole against Burkholderia pseudomallei in planktonic and biofilm forms, through broth microdilution and resazurin-based viability staining, respectively. In planktonic growth, the strains were susceptible to the drugs, while in biofilm growth, significantly higher antimicrobial concentrations were required, especially for ceftazidime and imipenem, surpassing the resistance breakpoints. These results highlight the importance of the routine evaluation of biofilm antimicrobial susceptibility.