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Nuclear receptor SHP inhibition of Dnmt1 expression via ERRγ.

FEBS letters (2011-04-05)
Yuxia Zhang, Li Wang
ABSTRACT

We describe a transcriptional mechanism regulating the expression of Dnmt1 by nuclear receptors. We show that ERRγ functions as a transcriptional activator of mouse and human Dnmt1 expression by direct binding to its response elements (ERE1/ERE2) in the dnmt1/DNMT1 promoters. The induction of Dnmt1 by ERRγ is repressed by SHP through SHP inhibition of ERRγ transactivity, diminishing ERRγ recruitment to the Dnmt1 promoter, and altering the conformation of local chromatin from an active mode by ERRγ to an inactive mode. Our study provides the first evidence for nuclear receptor mediated regulation of Dnmt1 expression through ERRγ and SHP crosstalk.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-dimethyl-Histone H3 (Lys4) Antibody, Upstate®, from rabbit
Sigma-Aldrich
Anti-acetyl-Histone H4 Antibody, serum, Upstate®
Sigma-Aldrich
ChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set, serum, from rabbit
Sigma-Aldrich
Anti-acetyl-Histone H3 Antibody, from rabbit