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GE17-0060-01

Sephadex® G-100

Cytiva 17-0060-01, pack of 100 g

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About This Item

UNSPSC Code:
23151817
NACRES:
NA.56

feature

autoclavable In wet form (pH 7.0) at 120°C for 30 min

packaging

pack of 100 g

manufacturer/tradename

Cytiva 17-0060-01

matrix

cross-linked dextran

particle size

100-310 μm (wet)
40-120 μm (dry)

working range

2-10

General description

Sephadex® G-100 is useful for desalting and buffer exchange of very large biomolecules.

Application

Sephadex® is a gel filtration medium prepared by crosslinking Dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and Low operating pressures are required.

Features and Benefits

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Classic gel filtration medium.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.

Column Packing and Preparation for Size Exclusion Chromatography

Related Content

Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography

Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.

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