G5760

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

Type XXIII, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret), ≥2.0 mg/mL Biuret

• Synonym(s): G-6-P-DH
• CAS Number: 9001-40-5
• Enzyme Commission number: 1.1.1.49 (BRENDA, IUBMB)
• EC Number: 232-602-6
• MDL number: MFCD00081656
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bacterial (Leuconostoc mesenteroides)
• Quality Level: 200
• type: Type XXIII
• form: ammonium sulfate suspension
• specific activity: 550-1,100 units/mg protein (biuret)
• mol wt: 54 kDa
• concentration: ≥2.0 mg/mL Biuret
• foreign activity: 6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%; PGI ≤0.01%
• storage temp.: 2-8°C

Description

General description

Research area: Cell Signaling

Glucose-6-phosphate dehydrogenase (G6PD) is found in the cytoplasm of all cells. The G6PD enzyme is encoded by the Gd gene, located on the long arm of the X chromosome.[1]Glucose-6-phosphate Dehydrogenase (G6PD) from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD⁺) or nicotinamide adenine dinucleotide phosphate (NADP⁺).[2] It corresponds to a molecular weight of 54 kDa and exists as a homodimer.[3]

Application

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used:

• as a component of reaction mixture for assaying mannose- and glucose-6-phosphate activity[4]
• as a component of reaction mixture in the nevirapine inhibition studies in human hepatic microsomes[5]
• in the glucose-phosphorylating activity of chloroplast extracts[6]
• as a model to test the effect of seed protein fractions on enzyme protection during dehydration.[7]
• in assays for nicotinamide adenine dinucleotide and tissue pyridine nucleotides.[8]

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Glucose-6-phosphate dehydrogenase (G6PD) is a housekeeping enzyme that protects cells from damage caused by reactive oxygen species by supplying substrates that help prevent oxidative harm.[1] G6PD from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD⁺) or nicotinamide adenine dinucleotide phosphate (NADP⁺) [2] as the first step in the pentose phosphate pathway. Lysine 21 in the Glucose-6-phosphate Dehydrogenase (G6PD) is crucial for binding NAD⁺.[2] G6PD uses NAD⁺ or NADP⁺ based on the catabolic or anabolic metabolic pathway.[3]G6PD deficiency can lead to acute hemolysis, neonatal jaundice, or severe chronic non-spherocytic hemolytic anemia.

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Physical form

Suspension in 2.7 M (NH₄)₂SO₄ solution containing 42 mM Tris and 0.8 mM MgCl₂

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves